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۱Using Biochemical and Simple Sequence Repeats (SSR) Markers to Characterize (Ficus carica L.) Cultivars
اطلاعات انتشار: World Applied Sciences Journal، بيست و نهم،شماره۳، ۲۰۱۴، سال
تعداد صفحات: ۹
The common fig (Ficus carica L.) trees are one of the earliest cultivated fruit–bearing trees. It is a moderately important world crop, with an estimated annual fruit production of 1,027,194. The aim of the study was to select the most efficient markers to propose a molecular key for seven fig cultivars identification. Genetic similarity matrix was generated on the basis of Nei and Li’s coefficients. The coefficients were used to makeclusters using un–weighted pair group method of arithmetic means (UPGMA) which separated the studied seven fig cultivars into two main groups depending on results PPO, POD isozymes and SSR–PCR combination. The first group I included Katoda cultivar (similarity range 0.43 to 0.92). However, the second group (II), the cultivars fell into three sub–groups closely related (a, b and c). Sub–group (a) (similarity range 0.36 to 0.81)contained on Sultany and Black Mission. Sub–group (b) (similarity range 0.41 to 0.75) included White Acdy, Aboudi and Conadria. Sub–group (c) consisted of Gizy (similarity range 0.36 to 0.81). Consequently, this study suggests the PPO and POD markers are suitable, but SSR marker is the most efficient for genetic variability and fig cultivars fingerprinting. In addition, genetic diversity could be used for rational design of breedingprograms, conservation of local germplasm and management of fig genetic resources.
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