مقالههای A Hatami
توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقالههای نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده میشوند.
اطلاعات انتشار: Research in Pharmaceutical Sciences، نهم،شماره۱(پياپي ۲۲)، ۲۰۱۴، سال ۰
تعداد صفحات: ۷
Chamomile (Matricaria chamomilla L.) is a widely used medicinal plant possessing several pharmacological effects due to presence of active compounds. This study describes a method of using ultra performance liquid chromatography (UPLC) coupled with photodiode array (PDA) detector for the separation of phenolic compounds in M. chamomilla and its crude extracts. Separation was conducted on C18 column (150 mm × 2 mm, 1.8 µm) using a gradient elution with a mobile phase consisting of acetonitrile and 4% aqueous acetic acid at 25°C. The method proposed was validated for determination of free and total apigenin and apigenin 7–glucoside contents as bioactive compounds in the extracts by testing sensitivity, linearity, precision and recovery. In general, UPLC produced significant improvements in method sensitivity, speed and resolution. Extraction was performed with methanol, 70% aqueous ethanol and water solvents. Total phenolic and total flavonoid contents ranged from 1.77 to 50.75 gram (g) of gallic acid equivalent (GAE)\100 g and 0.82 to 36.75 g quercetin equivalent (QE)\100 g in dry material, respectively. There was a considerable difference from 40 to 740 mg\100 g for apigenin and 210 to 1110 mg\100 g for apigenin 7–glucoside in dry material.
اطلاعات انتشار: Research in Pharmaceutical Sciences، نهم،شماره۵(پياپي ۲۶)، ۲۰۱۴، سال ۰
تعداد صفحات: ۸
In this study, caffeic acid (CA) and its three derivatives including 3–caffeoylquinic acid (3–CQA, neochlorogenic acid), 4–caffeoylquinic acid (4–CQA, cryptochlorogenic acid), and 5–caffeoylquinic acid (5–CQA, chlorogenic acid) were identified in Bupleurum chinense aerial parts using reverse–phase high–performance liquid chromatography (RP–HPLC) with photodiode array (PDA) detector, reference compounds and chemical reactions. Separation was performed on a C18 column using gradient elution with 4% (v\v) aqueous acetic acid and acetonitrile as mobile phase at ambient temperature. In addition, the flavonoid aglycones were characterized and quantified after acid hydrolysis of the plant material. The flavonols profile showed quercetin (0.36 g per 100 g), kaempferol (1.11 g per 100 g) and isorhamnetin (0.16 g per 100 g). Total phenolic and total flavonoid contents ranged from 7.3 to 18.7% and 0.58 to 2.72% in dry plant material, respectively.
نمایش نتایج ۱ تا ۲ از میان ۲ نتیجه