توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Detection of salmonella in raw poultry meat by PCR and traditional culture method
نویسنده(ها): ، ،
اطلاعات انتشار: چهارمین همایش ملی بیوتکنولوژی ایران، سال
تعداد صفحات: ۴
Contaminated poultry meat has been identified as one of the principal foodborne sources of salmonella.Traditional culture method for detection of salmonella requires 4–6 days to processing samples. PCR has became the potential of a powerful alternative in microbiological diagnostics due to its rapidity and accuracy. In this study a survey was carried out to determine the prevalence of salmonella in raw broiler carcasses. A total of 60 neck skin swab samples were taken from broiler carcasses after the chilling stage of processing. The presence of salmonella was assessed by traditional culture method and confirmed by serotyping and PCR amplificaton of invA gene. Incorporation of a routin PCR test in conjunction with standard culture,could be effective in providing a more accurate profile of the prevalence of this pathogen in broiler carcasses.<\div>

۲Comparison of Most Probable Number–PCR and Real–Time PCR Methods for Quantitative Detection of Listeria Monocyotgenes in Milk
نویسنده(ها): ، ، ،
اطلاعات انتشار: همایش ملی علوم و فناوریهای نوین در صنایع غذایی، سال
تعداد صفحات: ۱۸
Detection and enumeration of Listeria monocytogenes as a majorfood–borne pathogen are of increasing interests, particularly after recent occasional outbreaks. Conventional culture based techniques for detection of L. monocytogenes are laborious, time consuming and are not very sensitive. To evaluate other quantitative methods which give earlier results two methods, Most Probable Number–Polymerase Chain Reaction (MPN–PCR1) and real–time PCR, have been evaluated by using milk as matrix media.Materials and methods:From pure cultures of L. monocytogenesand and 8 other background bacteria approximately to 107 and 101 CFU\ml suspensions were prepared. The 3 test tubes MPN sets were prepared from 101 CFU\ml of bacterial mix suspension in listeria enrichment broth. MPN sets were prepared. After incubation turbid MPN tubes were subjected to DNA isolation and applied to PCR (MPN–PCR). Ten–fold dilutions were prepared from genomic DNA isolate from bacterial mix suspension and L. monocytogenesstock suspension (corresponding approximately to 107–10–2 genomic DNA copy\ml) for real–time PCR assay as testing samples and standard samples respectively. MPN–PCR and real–time PCR assays were conducted in triplicate using appropriate primers for the prfAgene.Results:The detection limit of MPN–PCR was estimated as 101 CFU\ml. These estimations were more precise than real–time PCR results which just could detect bacteria in concentrations of ≥104 bacteria per milliliter.Conclusion:According to the findings of this study MPN–PCR can be used both as a detection and also an enumeration method to assess the presence of L. monocytogenes in milk samples.<\div>

۳Using multiplex–PCR assay in identification of Escherichia coli O157:H7 isolated from hamburger samples in Mashhad, Iran
نویسنده(ها): ، ، ،
اطلاعات انتشار: Journal of Food Science and Technology، سال
تعداد صفحات: ۷
In this study a number of 100 hamburger samples which were produced industerially from different batches were collected randomly from suprermarkets in Mashhad city, during the autumn months of 2006. For isolation of the bacteria, samples were, firstly enriched in modified trypticase soy broth containing novobiocin, followed by plating on sorbitol Mac Cankey agar supplemented with cifixime and potassium tellurite. Consequently the suspected non sorbitol fermenting (NSF) colonies were confirmed by biochemical tests as Escherichia coli and then employed for multiplex–PCR assay, using primers specific for O157 and H7 antigens gene. The m–PCR assay employed in this study may be a possible alternative to immunological assays which detects somatic and flagellar antigens. In this study, 9 NSF Escherichia coli colonies were isolated, and in multiplex–PCR assay two samples (4%) were confirmed as Escherichia coli O157:H7
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