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۱Isolation and Identification of Thermophillic Actinomycetes from Hot Water Springs from Azad Jammu and Kashmir Pakistan for the Production of Thermophillic Amylase
اطلاعات انتشار: World Applied Sciences Journal، سي ام،شماره۳، ۲۰۱۴، سال
تعداد صفحات: ۵
Three samples of soil were collected from the hot springs from Kotli Azad Jammu and Kashmir. Two samples were collected from the West and North Banks respectively and the third one was collected from inside the hot water of the spring. For serial dilutions 1g soil was dissolved in 100 ml of sterilized distilled water. The soil solution was further diluted up to thousand times. Out of these dilutions 10 ml sample was taken in test tube and kept at 90°C in an oven to give heat shock for duration of 15 minutes. The ml dilution was spread on the AGS medium. Out of 20 strains six giving higher zones of hydrolysis (N3, W1, W3 U1, U2 and U4) were inoculated nutrient starch agar slants. Four strains of bacteria N3, W1, U1 and U4 showing higher zones of hydrolysis i.e. 3.6, 4.2, 3.8 and 3.6 mm respectively. Strains N3, U1 and U2 were found to be bacillus and W1 was identified as Actinomycetes. On the basis of morphological and physiological tests as determined by Bergeys manual of bacteriology W–1 was identified as Thermoactinomyces sacchari. Identification was also done by PCR for which the forward and the reverse primers were prepared by the fermentos company USA. In PCR 2,3,6,8 lane gave positive results and band formation. Morphological identification and the biochemical tests confirmed that the strain was Thermoactinomyces sacchari so the strain was selected for further study. Different types of media were utilized for the maximum production. Growth of Actinomycete and amylase production was optimized on four different nutrient media. Highest growth and amylase activity (3.882 OD and 132.3 AU, respectively after 72 hours at 60°C) were obtained by using medium–4 and it is also called as AGS medium.

۲In vitro Approaches of Primula vulgaris Leaves and Roots Extraction against Human Pathogenic Bacterial Strains
اطلاعات انتشار: World Applied Sciences Journal، سي ام،شماره۵، ۲۰۱۴، سال
تعداد صفحات: ۶
Primula is a medicinal plant bearing flowers which belongs to family Primulaceae. These are low growing herbs. Methanol and cold water extraction methods were used for leaves and roots extractions of Primulavulgaris. Antimicrobial activity ofleaves and roots extracts was evaluated by well diffusion method. Results revealed that P. vulgaris leaves extractexhibit excellent inhibitory effects against Escherichia coli that showed zone of inhibition 18 mm with methanol extract and 14 mm with cold water extract and Pseudomonas aeruginosa showed zone of inhibition 14 mm with methanol extract and 13 mm with cold water extract. Roots extract of P. vulgaris also exhibited inhibitory effects against E. coli showed zone of inhibition 13 mm with methanol extract and 16 mm with cold water extract and P. aeruginosa showed zone of inhibition 13 mm with methanol extract and 14mm with cold water extract while Staphylococcus aureus did not show any inhibitory zone with leaves or roots extracts. This research summarized that leaves and roots extracts of P. vulgaris contains a brought range of antimicrobial activity against the microorganism. Advance studies should carry out to find out the precise method of action by which extracts exert their antimicrobial effect to recognize which can be used in drug development.
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