مقالههای Ahmad Arzani
توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقالههای نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده میشوند.
۱Functional Analysis of a Pomegranate (Punica granatum L.) MYB Transcription Factor Involved in the Regulation of Anthocyanin Biosynthesis
نویسنده(ها): Ghazale Khaksar، Badredin Ebrahim Sayed Tabatabaei، Ahmad Arzani، Cyrus Ghobadi، Esmaeil Ebrahimie
اطلاعات انتشار: Iranian Journal of Biotechnology، سيزدهم،شماره۱(پياپي ۴۹)، ۲۰۱۵، سال ۰
تعداد صفحات: ۹
Background: Pomegranate fruit (Punica granatum L.) is a rich source of anthocyanin pigments resulting in vibrant colours and anti–oxidant contents. Although the intensity and pattern of anthocyanin biosynthesis in fruit are strongly influenced by R2R3–MYB transcription factors, little is known about the regulation and role of MYB in anthocyanin pathway of pomegranate.Objectives: The present study was conducted to elucidate the relationship between the expression of MYB transcription factor and the anthocyanin accumulation during the colour development phase of pomegranate fruits.Materials and Methods: In this work, R2R3–MYB transcription factor (PgMYB) was isolated and characterized from pomegranate skin through RACE–PCR. The expression of PgMYB gene was monitored in three distinct pomegranate accessions with distinctive skin colour and pattern by semi–quantitative RT–PCR.Results: The results indicated a strong association between skin colour in mature pomegranate fruits with the PgMYB transcripts. The highest expression level of PgMYB gene was observed in Poost Siyah Yazd (dark purple skin) throughout the ripening process. Furthermore, comparison of PgMYB amino acid sequences with those of R2R3–MYB family in grapevine, eucalyptus, peach, cacao, populus and Arabidopsis demonstrated that this protein shares high similarity (75–85% amino acid identity) with their conserved MYB domain. Computational structure prediction of PgMYB showed that the three conserved amino acids (Asn, Lys and Lys) are present in the same position of the MYB domain. Conclusions: It is speculated that PgMYB gene influences the fruit colour and could be used to improve the accumula–tion of anthocyanin pigments in the pomegranate fruit.
اطلاعات انتشار: Journal of Plant Molecular Breeding، اول،شماره۲، ۲۰۱۳، سال ۰
تعداد صفحات: ۱۶
Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. This research was conducted to assess genetic variation induced by gamma radiation in M2 and M3 mutant lines of canola (Brassica napus L.) by SSR and morphological characteristics and to identify useful mutants in terms of agronomic traits. Sixty–two mutant lines derived from gamma mutagenesis and their wild–type progenitors (‘RGS003’ and ‘Sarigol’ cvs) were used. Twenty–five polymorphic SSR primers were used in this study. Results of cluster analysis based on both morphological traits comprising plant height, days to flowering, days tomaturity, number of pods\plant, number of seeds\pod, 1000–seed weight and seed yield\plant and SSR data revealed a separate grouping of mutant lines from control cultivars. SSR data analysis of mutant lines and controls demonstrated a considerable genetic variation among mutant lines, where 83% of primers generated polymorphic bands with 3.32 alleles per locus. The genetic distance calculated between mutant lines and their controls indicated a significant difference between mutant lines and controls. Although both morphological and SSR markers successfully discriminated mutant lines from controls, SSR primers could further discriminate between the mutant lines derived from the related cultivar. Mutant lines 24 derived from ‘RGS003’ and 16 and 26 from ‘Sarigol’ were considered as superior for breeding canola, which could be utilized in future genetic and breeding programs. Distinct classification of genotypes based on agromorphological and SSR data in the present study implies that morphological and SSR markers reflected different aspects of genetic variation among mutant lines.
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