توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Agrobacterium–Mediated Transformation of Pomegranate (Punica granatum L.) ‘Yousef Khani’ Using the gus Reporter Gene
اطلاعات انتشار: International Journal of Horticultural Science and Technology، اول،شماره۱، Spring and Summer ۲۰۱۴، سال
تعداد صفحات: ۱۱
In this study, an efficient Agrobacterium–mediated transformation method was developed for pomegranate (Punica granatum L.), a difficult–to–transform plant. In vitro shoot segments were inoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β– glucuronidase (gus) gene as a reporter. After 28 d in WPM selection medium containing 50 mg L–1 kanamycin, 59 new shoots proliferated. gus analysis was performed on these putative transgenic shoots, of which 32 stained positive. Positive staining shoots were cut and cultured in selection medium for 2 subsequent subcultures until final gus analysis. After three months of the selection period, 6 putative transgenic shoots were obtained. Presence of the gus and nptII genes was confirmed by polymerase chain reaction. Southern blot analysis confirmed that TDNA was stably integrated into the genome of three out of six PCR–positive plants. The transgenic plants were rooted and successfully acclimatized.

۲Morphological Changes in Response to Drought Stress in Cultivated and Wild Almond Species
اطلاعات انتشار: International Journal of Horticultural Science and Technology، اول،شماره۱، Spring and Summer ۲۰۱۴، سال
تعداد صفحات: ۱۴
This study was undertaken to identify morphological changes in young seedlings of 5 Iranian almond species (Prunus dulcis, P. eburnea, P. eleagnifolia, P. haussknechti, and P. scoparia) under polyethylene glycol–induced drought stress. Drought stress caused a significant reduction in plant growth parameters such as fresh and dry weights of plant organs, leaf number, total leaf area, and leaf relative water content in all almond species. Specific leaf weight also increased significantly in drought–treated plants compared to control. No significant changes in shoot length, individual leaf area, leaf dimension (length and width), or stomatal size and frequency were observed in response to drought treatments. P. eburnea had the highest relative water content among the species and showed the smallest decrease in fresh and dry weights of organs and greatest decrease in leaf number and total leaf area (the most reduction in transpiration area) as an adaptive mechanism to drought stress.

۳Paclobutrazol–Induced Biochemical Changes in Pomegranate (Punica granatum L.) cv. ‘Malas Saveh’ under Freezing Stress
اطلاعات انتشار: International Journal of Horticultural Science and Technology، اول،شماره۲، Autumn ۲۰۱۴، سال
تعداد صفحات: ۱۰
Freezing injury is an important limiting factor in the production of pomegranate in Iran.The aim of the present study was to evaluate the effect of paclobutrazol (PBZ) on cold hardiness of pomegranate (Punica granatum L.) cv. ‘Malas Saveh’. Different concentrations of PBZ including 0 (control), 31, 62, 125 or 250 mg L–1 were sprayed on one–year–old plants in August and the electrolyte leakage of their stems was measured at three acclimation stages (November, January, and March). PBZ treatments, especially at 125 and 250 mg L–1 concentrations increased cold hardiness, and corresponding soluble carbohydrates and proline contents. The highest variation in freezing tolerance was observed between control and PBZ–treated plants in January, and the lowest found in November. Irrespective of PBZ treatment, correlations between cold hardiness and soluble carbohydrate concentrations were stronger, compared to proline. Soluble carbohydrates were higher in January, associated with deep dormancy, whereas the maximum proline content was detected in March, at deacclimation stage. Results suggest that PBZ application can reduce low temperature–induced dysfunction of cell membrane through increasing soluble carbohydrates and proline contents.

۴Effect of postharvest calcium chloride treatment on the storage life and quality of persimmon fruits (Diospyros kaki Thunb.) cv. ‘Karaj’
نویسنده(ها): ، ، ،
اطلاعات انتشار: International Journal of Horticultural Science and Technology، دوم،شماره۱، Spring and Summer ۲۰۱۵، سال
تعداد صفحات: ۱۲
The objective of this study was to evaluate the storage life and quality of persimmon (Diospyros kaki Thunb.) fruit cv. ‘Karaj’ stored at 0°C for 4 months after postharvest calcium chloride treatment. Fruit weight loss, fruit firmness, total soluble solids, titratable acidity, total phenolic content, soluble tannin content, chilling injury, antioxidant activity, electrolyte leakage, and malondialdehyde content were measured in 0, 15, 30, 45, 60, 75, 90, 105, and 120 days after storage. Peroxidase and catalase enzyme activities were also determined at the end of the storage. Fruits were dipped in the solutions containing 0.5, 1, and 2% (w\v) CaCl2 as well as distilled water only as the control. Results showed that the fruit weight loss and chilling injury significantly decreased by CaCl2 treatments compared with the control. CaCl2 treatments also increased fruit firmness and catalase and antioxidant activities, whereas they reduced MDA content, EL, and peroxidase activity during 4 months of cold storage. The results indicated that CaCl2 application influenced TPC and soluble tannin compared with the control but had no significant effect on TA. The best result was obtained from the 2% CaCl2 treatment in almost all the studied parameters. In general, postharvest CaCl2 treatment of persimmon could decrease chilling symptoms during the cold storage.
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