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۱Improvement of maize (Zea mays L.) anther culture embryogenesis and direct regeneration by different plant growth regulators and gelling agent
اطلاعات انتشار: Journal of Applied Biotechnology Reports، اول،شماره۱، Winter ۲۰۱۴، سال
تعداد صفحات: ۵
Androgenesis via anther culture or microspore culture is one of the current methods for producing haploid and double haploid plants in maize. To use of this method in maize breeding program it should be able to regenerate enough plantlets. Anthers culture usually is carried out indirectly via callus induction and regeneration on at least two different media. In this study a responsive genotype, ETH–M82, using a new single culture medium was used for embryogenesis and regeneration. We tested different growth regulators (2,4–D; kinetin; NAA & IAA) in modified YP medium. After6–week, direct regeneration on some treatments was observed. The highest frequency of direct formation of plantlets (in 100 anthers) occurred on medium supplemented with 2mgl–1 IAA and 2mgl–1 kinetin (4%).Best results with an average of 3.1plantlets in replication were obtained with the medium solidified with agar, while in difcobactoagar only 1.4 of plantlets in replication was produced. This experiment suggested that agar and plant growth regulators in the medium were beneficial for producing embryo and plantlet from maize anthers.

۲Study of Paraoxonase –1 Gene Polymorphism in a Healthy Population of
اطلاعات انتشار: Journal of Applied Biotechnology Reports، اول،شماره۲، Spring ۲۰۱۴، سال
تعداد صفحات: ۵
Human serum paraoxonase (HuPON1: EC 3.1.8.1), a calcium–dependent esterase, is synthesized in the liver and widely distributed in tissues including liver, kidney, intestine, and serum, where it is associated exclusively with high–density lipoprotein. Human paraoxonase–1 plays an important role in prevention of atherosclerosis and also protection against organophosphate–induced neurotoxicity. Paraoxonase–1 shows 2 common polymorphisms: Q\R at position 192 and M\L at position 55. In this study, paraoxonase–1 192 and 55 polymorphisms were investigated in 64 healthy Iranian individuals. Genomic DNA was isolated from whole blood by the Bartlett method, and paraoxonase–1 genotypes were determined by polymerase chain reaction amplification followed by restriction isotyping and gel electrophoresis. The chi–square test was used to evaluate the Hardy–Weinberg equilibrium. The genotype frequencies for paraoxonase 1–Q192R­ were approximately 47% (QQ), 41% (QR) and 12% (RR) and for paraoxonase–1 M55L, 44% (LL), 44% (ML) and 12% (MM). Thus, the frequency of alleles R, L, Q, and M were 0.33, 0.66, 0.67, and 0.34 respectively. In conclusion, the frequencies of paraoxonase–1 192 and 55 polymorphisms in this group of Iranian population were different from those seen in other Asian populations from Japan and China but similar to European (Caucasians).

۳Phenol–stacked carbon nanotubes: A new approach to genomic DNA isolation from plants
اطلاعات انتشار: Molecular Biology Research Communications، سوم،شماره۳، ۲۰۱۴، سال
تعداد صفحات: ۱۰۹
Extraction of intact quality DNA from plant tissues, especially those rich insecondary metabolites, is often challenging. Literally, hundreds of different DNAisolation protocols from various plant species have been published over the last decades.Although many commercial DNA isolation kits are convenient and designed to be safe,their cost and availability cause limitations in small molecular labs in many developingcountries. In nearly all protocols and DNA isolation kits, phenol and chloroform areused to precipitate various classes of impurities. However, phenol is partially soluble inwater, resulting in the co–existence of proteins in upper (aqueous) phases. Thisphenomenon results in the contamination of the nucleic acids and low quality DNA.Nanotechnology advances have helped many areas of molecular biology such as thedevelopment of new diagnosis and purification kits. In this study, for the first time, wereport a different approach to isolate DNA from plants based on carbon nanotubes(CNTs). The results show that the phenol reagent stack on CNTs can effectively removeproteins, polysaccharides and other polyphenol constituents. The A260\A280nmabsorbance ratios of isolated DNA samples were 1.9 and 1.8 for chamomile and opiumplants, respectively, indicating the high purity of the isolated DNA. DNA yield wasmore than two times the standard Doyle and Doyle method. Furthermore, the isolatedDNA proved amenable to PCR amplification, using Random Amplified PolymorphicDNA (RAPD) analysis.
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