توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Nano–bio Hybrid Material Based on Bacteriorhodopsin and ZnO for Bioelectronics Applications
اطلاعات انتشار: International Journal Of Bio-Inorganic Hybrid Nanomaterials، سوم،شماره۴، winter ۲۰۱۵، سال
تعداد صفحات: ۷
Bioelectronics has attracted increasing interest in recent years because of their applications in various disciplines, such as biomedical. Development of efficient bio–nano hybrid materials is a new move towards revolution of nano–bioelectronics. A novel nano–bio hybrid electrode based on ZnO–protein for bioelectronics applications was prepared and characterized. The electrode was made by covalent attachment of bacterorhodopsin (bR) on to the ZnO substrate. The protein was attached to the ZnO nanoporous film coated on FTO glass with and without linker. In the immobilization method by adsorption without linker, protein was bonded to ZnO via electrostatic interactions and in the immobilization method protein was attached covalently. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were employed to investigate the surface features of the ZnO thin film and attached protein. ATR–FTIR was used to confirm the protein attachment.

۲Evaluation of Immobilized Bacteriorhodopsin’s Function by Laser Irridiation
اطلاعات انتشار: Biomacromolecular Journal، اول،شماره۲، Autumn ۲۰۱۵، سال
تعداد صفحات: ۸
Bacteriorhodopsin (BR) is a retinal protein that is a light–driven proton pump and has an important role in photosynthesis in archaebacterium Halobacterium salinarum. The BR molecule absorbs light and photochemical changes occur in it, and different intermediates will be produced in its photochemical cycle that some of them like P and Q intermediates have a long half–life. There have been many efforts to immobilize BR for constructing data storage devices. In this study the BR suspension–contained film has been immobilized on a polycarbonate and the effects of green and red lasers on it (in different times and temperatures) have been investigated by UV spectrophotometer method, The modified surfaces were characterized by ATR–FTIR and AFM techniques. The results show that with used condition for immobilization of BR on polycarbonate, the 0 and 1 bite that relate to BR and P, Q intermediate, are formed. The red and green lasers convert BR to O and, P or Q intermediates respectively that could be used instead of 0 and 1 bites in popular compact disks.

۳Immobilization of Subtilisin Carlsberg on Modified Silica Gel by Cross–linking and Covalent Binding Methods
اطلاعات انتشار: Biomacromolecular Journal، دوم،شماره۱، Summer ۲۰۱۶، سال
تعداد صفحات: ۶
Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3–aminopropyltriethoxysilane then the protease enzyme was immobilized on the modified silica by glutaraldehyde cross–linking method and the immobilized enzyme’s activity was maintained for more than 40 days. Measuring the free subtilisin carlsberg enzyme activity and immobilized enzyme was performed according to the Lowry method. In another part, the effects of different pHs and temperatures on free and immobilized protease were evaluated. The immobilized protease activity was measured in temperature range between 25–75◦C and pH range 6.5–12. The absorption was read in 660nm. It is shown that the optimum temperature for immobilizaed enzyme is 50°C. The results showed that immobilized protease is more stable than free protease.
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