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۱Acute toxicity of Euphorbia turcomanica on Aphanius dispar
نویسنده(ها): ، ، ،
اطلاعات انتشار: International Journal of Aquatic Biology، سوم،شماره۵، Oct ۲۰۱۵، سال
تعداد صفحات: ۶
Piscicidal and molluscicidal activities of aqueous extracts of many members of the family Euphorbiaceae are well–known, but the toxicity potential of Euphorbia turcomanica was not yet studied on any aquatic animals. An acute toxicity test was performed by using a four–day static renewal test to determine the LC50 value of dried powder of E. turcomanica for the euryhaline fish, Aphanius dispar. The LC50 values at various exposure periods are 0.177±0.039 g\L for 24 hrs, 0.131±0.030 g\L for 48 hrs, 0.073±0.018 g\L for 72 hrs, and 0.052±0.013 g\L for 96 hrs. The toxicity of dried powder of E. turcomanica exhibits a positive correlation between fish mortality and exposure periods. As this is the first report about toxicity of E. turcomanica on A. dispar, the results could be only compared to that of other Euphorbiaceae as well as other fishes. It is concluded that the toxicity potential of E. turcomanica is comparable and close to that of well–documented Euphorbiaceae. It has been suggested that E. turcomanica products cannot be used directly in fish–inhabiting water reservoirs.

۲Amelioration of cadmium–induced changes in biochemical parameters of the muscle of Common Carp (Cyprinus carpio) by Vitamin C and Chitosan
اطلاعات انتشار: International Journal of Aquatic Biology، سوم،شماره۶، Dec ۲۰۱۵، سال
تعداد صفحات: ۱۰
The aim of this study was to investigate the effects of administering antioxidants, including vitamin C and chitosan on oxidative stress markers in muscle as edible tissues of Cyprinus carpio exposed to cadmium chloride. In this experiment, by exposing to 0.2 mg\L cadmium chloride for 21 days, fish were fed a normal diet, diet containing chitosan (1000 mg\kg diet), vitamin C (1000 mg\kg diet) or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA) as well as biochemical parameters, including the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and acetylcholinesterase (AChE) were measured. Fish exposure to cadmium chloride significantly increased AST, LDH, CPK, catalase, and MDA activity, while it significantly decreased AST and AChE activity, and levels of total antioxidant in muscle cells. Administration of chitosan or vitamin C alone or in combination with each other to fish exposed to cadmium chloride was effective in regulating ALT, CPK, and catalase activity. Although administration of vitamin C and chitosan caused a significant decrease in MDA, AST and LDH, these enzymes were still significantly higher than those in the control group. Administration of vitamin C and chitosan had no significant effects on the activity of AChE and levels of total antioxidant. Although, chitosan alone could not prevent oxidative stress damages in muscle tissues of cadmium–treated fish, administration of vitamin C combined with chitosan may increase the efficiency of antioxidant defense system and improve the detoxification system in the muscles of fish exposed to cadmium chloride.
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