توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Development of transgenic mice harboring ovine beta lactoglobulin–calcitonin transgene
اطلاعات انتشار: Iranian Journal of Biotechnology، دوم،شماره۳(پياپي ۷)، ۲۰۰۴، سال
تعداد صفحات: ۶
Expression of foreign proteins in mammalian milk is becoming a widespread strategy for high–level production of recombinant pharmaceuticals, especially those with the most complex post–translational modifications. A gene construct was generated, consisting of 10.7 kbp of the ovine beta–lactoglobulin (oBLG) gene including its promoter and 3 flanking region with the calcitonin coding sequences inserted in–frame into the oBLG fifth exon. The gene construct was purified using CsCl gradient, released from vector, and gel–purified. It was microinjected into fertilized mouse oocytes. These oocytes were then transferred to pseudo–pregnant foster mice. The pups born from foster mice were genotyped using PCR, slot blot, and Southern blot techniques. Among 9 mice which showed positive PCR results, only 6 mice transmitted the transgene to the next generation. Therefore, 6 transgenic lines were established which stably transmitted their transgene to their progeny.
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