مقالههای Ahmed B. Barakat
توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقالههای نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده میشوند.
۱Molecular Epidemiology of Human Adenoviruses and Rotaviruses as Candidate Viral Indicators in the Egyptian Sewage and Water Samples
اطلاعات انتشار: World Applied Sciences Journal، بيست و هفتم،شماره۱۰، ۲۰۱۳، سال ۰
تعداد صفحات: ۱۳
The prevalence of human rotavirus and adenovirus in sewage and water samples before and after treatment steps and also in clinical samples was compared in this study to determine the most prevalent virus and the more resistant one to treatment processes. The prevalence percentage of rotaviruses in sewage samples collected from raw sewage of Zenin wastewater treatment plant (WWTP) from July 200 to June 2011 was 16.7% (4\24) while the prevalence percentage of adenoviruses in the same raw sewage samples was 91.7% (22\24). The prevalence percentage of rotaviruses in Nile water samples collected from El– Giza water treatment plant (WTP) from July 2009 to June 2011 was 29.2% (7\24) while the prevalence percentage of adenoviruses in the same Nile water samples was 66.7% (16\24). Rotavirus could be only detected and quantified as genome copies and infectious units in the peak months of the virus in autumn and winter. Adenovirus genome copies and infectious units were higher in winter than in the other months but considerable numbers of both of them were quantified all the year. The means of adenovirus genome and infectious units removals were 3.5±1.1 log10 and 2.8±0.8 log10 respectively while the means of rotavirus genome and infectious units removals were 3.75±0.5 log10 and 3±1.2 log10 respectively after treatment processes of Zenin WWTP. The means of adenovirus genome and infectious units removals were 2.3±0.9 log10 and 2.1±0.9 log10 respectively while the means of rotavirus genome and infectious units removals were 2.86±1.1 log10 and 2.57±1.1 log10 respectively after treatment processes of El–Giza WTP. Rotavirus was the most prevalent in diarrheal samples 31.8% (35\110), while adenovirus was detected in 18.1% (20\110) of the samples. Sequence analysis of positive adenovirus samples indicated that 19 samples (6 raw sewage, 5 Nile water and 8 clinical samples) showed similar sequences clustered with human adenovirus 41 strain 503804\KOL\2009 hexon gene with 94% homology and 5 samples 2 raw sewage and 3 Nile water samples) showed similar sequences clustered with human adenovirus 2 strain N377 \TW\05–2 hexon gene with 98% homology. As a conclusion, human adenovirus may be a suitable candidate viral indicator of human viral contamination in Egyptian water and sewage samples.
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