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۱Bioassay of Egyptian Propolis on Toxocara vitulorum Adult Worms
نویسنده(ها): ، ، ، ،
اطلاعات انتشار: World Applied Sciences Journal، سي و چهارم،شماره۳، ۲۰۱۶، سال
تعداد صفحات: ۷
Indiscriminate uses of chemical anthelmintics have resulted in the establishment of resistant parasites. Thus, this study aimed to evaluate the antiparasitic effect of Egyptian propolis ethanolic extract –as an alternative used drug– on adult Toxocara vitulorumin vitro. Adult worms were incubated for 24 h. in different concentrations of the extract (100, 50, 25, 12 and 6 mg\ml). The extract revealed anthelmintic efficacy and the mortality rate was concentration dependant. LC , LC and LC were 6.9, 12.5 and 53.4 mg\ml, respectively. 25 50 90 The mode of action of propolis ethanolic extract on adult worms was assessed bylight and scanning electron microscopy following 24 h. incubation. The disintegration of hypodermis, disorganization of muscle layers and retraction of lips, cuticle damage and distortion of excretory pore were detected and confirmed the nematicidal effect of Egyptian propolis. The overall findings of the current study confirmed effective and natural anthelmintic alternative to the more expensive drugs could be developed using Egyptian propolis.
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