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۱Characterization and Evaluation of Some Mung Bean Genotypes for Salt Tolerance
اطلاعات انتشار: World Applied Sciences Journal، سي و سوم،شماره۳، ۲۰۱۵، سال
تعداد صفحات: ۱۱
Total seed storage proteins extracted from 21 mung bean genotypes were separated using SodiumDodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS–PAGE). Seed storage proteins resolved thirty three protein polypeptide bands of diverse molecular weights ranging from 171 to 14 kDa. The 21 genotypes were grouped into three clusters depending on percent of homology. A total of 17 genotypes were grouped together in the frest cluster. This group of genotypes showed 100% homology when compared with each other. The second, other three genotypes, 10, 13 and 14 (numbers of genotypes) were also similar in seed protein profiles when compared with each other. These groups of genotypes showed 97% homology with the first group. The third cluster consisted of one genotype L19 which revealed high variations in terms of band number staining and molecular weight, the band thickness and sharpness exceptionally with genotype number 19 wasobserved when compared to other genotypes of tested mung bean. This genotype showed 24% homology with genotypes of the other clusters. The results depicted a low genetic diversity in the tested germplasm of the Egyptian origin (L1 to L11) compared with Indian origin (L12 to L21) based on seed storage proteins. Also the hydroponic experiments were used to study salt stress on the different genotypes of Vigna radiata (mung bean) plants in complete randomized block design with reliable growth and physiological characteristics. The results showed that different genotypes of mung bean exhibited distinct and significant response during screening for salt tolerance at seedling growth stage, under different salinity stress levels by NaCl treatment. The tolerant genotypes showed less reduction in length, Fresh and dry weight of shoot and root, membranestability, chlorophyll a and chlorophyll b contents, under high salinity level (200 mM NaCl) with respect to control plants. However, the susceptible genotypes showed greater reduction in the measured parameters under salinity stress. On the basis low and best performance of growth, physiological and biochemical of each genotypes under high salinity levels, total 6 genotypes L1, L13, L17, L18, L19 and L21 were selected from the studied genotypes and which will be screened further for the identification of most salt tolerant and susceptible genotypes to be used in breeding for the genetic improvement of mung bean for salt stress tolerance.
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