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۱Isozyme Markers for Response of Wild Potato Species to Potato Spindle Tuber Viroid Egyptian Isolate
اطلاعات انتشار: World Applied Sciences Journal، بيست و هفتم،شماره۸، ۲۰۱۳، سال
تعداد صفحات: ۱۳
The potato (Solanum tuberosum L.) is the fourth most important food crop worldwide. Potato Spindle Tuber Viroid (PSTVd) is devastating to potato crop and resistant cultivars are highly effective in controlling the disease. This research focused on screening wild potato species resistance to PSTVd. In addition, identify isozyme markers (PPO and POD) linked to the resistance loci from these wild potato accessions that can be used for marker–assisted selection in breeding programs. Eight wild potato species virus tested were screened to PSTVd response using peroxidase (POD) and polyphenol oxidase (PPO) isozymes. There are found free from potato viruses (Potato Virus X, Potato Virus Y and Potato Leaf Roll Virus) using DAS–ELISA. On the contrary, the eight species had different response to PSTVd based on disease severity and tomato cv. Rutgers index. Solanum etuberosum was immune while S. demissum was susceptible; S. sucrense and S. chacoense were resistant and S. stoloniferum, S. berthaultii, S. acaule and S. guerreroense were tolerant. These results were confirmed with by dot–blot hybridization. Two PPO isozyme markers (15.4% polymorphism) were recorded; one of them was detected in S. sucrense with Rf of 0.75 and another one in S. etuberosum with Rf of 0.87. Also, three POD–isozyme markers (33.3% polymorphism) were recorded; one of them was detected in S. guerreroense with Rf of 0.33, the second in PSTVd tolerant S. stoloniferum with Rf of 0.75 and the third in S. etuberosum with Rf of 0.85. The UPGMA cluster analysis showed a higher degree of relationship between immune species (S. etuberosum), tolerant species (S. stoloniferum, S. berthaultii, S. acaule and S. guerreroense) and resistant species (S. chacoense and S. sucrense) than susceptible species (S. demissum). Since, the closest relationships were observed between resistant species. In the case of immune species, the closest relationships were observed between it and tolerant species (S. guerreroense). On the other hand the lowest relationships were observed between immune species and susceptible species. Consequently, PSTVd resistant wild potato species could be used in breeding programmes by using as recurrent parents the susceptible high–quality potato cultivar and PSTVd resistant a wild potato species as donor parents.
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