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۱In–vitro Enhancement of Salinity Tolerance in Rice Using Cobalt Sulfate
اطلاعات انتشار: World Applied Sciences Journal، سي و يكم،شماره۷، ۲۰۱۴، سال
تعداد صفحات: ۱۰
This investigation was conducted to study the effects of cobalt sulfate on decreasing the negative salt stress effects. Calli of three Egyptian rice cultivars were obtained on LS medium supplemented with cobalt sulfate (5 mg\l), in addition to control treatment. The rate of callus proliferation increased with the presence of cobalt sulfate in the induction medium. Different treatments were made to study the effects of salinity, cobalt sulfate and the combination between them on the studied parameters (callus dry weight, free proline content, macronutrients, micronutrients and plantlets regeneration). Results showed that, under salinity stress, decreasing in dry weight and regeneration capacity, in contrast, an increasing in free proline accumulation was observed. The presence of cobalt sulfate decreased the negative impact of salt stress that observed in these indicators. Also, the levels of macro and micronutrients that affected by salt stress were not occurred in the presence of cobalt sulfate. RAPD studies were conducted using eight primers to find out the differences among them by occurrence of polymorphic bands due to the studied treatments. Distinguished specific bands identified salt stress and cobalt treatments effects on the studied genotypes. It could be concluded that, exposure to cobalt sulfate before exposure to salt stress led to reducing its harmful effects on the studied parameters.
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