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۱Najdi, Harri and Aradi Saudi Goat Breeds Possess Genetic Variation Required for Genetic Improvement
اطلاعات انتشار: World Applied Sciences Journal، بيست و ششم،شماره۷، ۲۰۱۳، سال
تعداد صفحات: ۶
Seven Inter–Simple Sequence Repeats (ISSR) primers were used to assess and characterize genetic diversity of Najdi, Harri and Aradi Saudi goat breeds. Thirty animals were selected randomly (5 males and 5 females per breed). These seven primers produced 138 ISSR loci (287 fragments). Amplified PCR fragments size ranged from 150 bp to 1250 bp. Most fragments were common in the three breeds. Across breeds percentage of polymorphic loci was 97.8%, where Harri breed had the largest percentage of polymorphic loci 51.03% followed by Najdi (42.03%) where Aradi had the smallest percentage (23.9%). All estimated parameters of genetic diversity such as observed number of alleles, effective number of alleles and polymorphism information index (PIC) showed high polymorphism across the studied loci and breeds. The mean coefficient of gene differentiation (Gst) was 0.59 indicating 29% of the total genetic diversity within breeds (Ht). Cluster analysis was carried out using unweighed pair group method with arithmetic averages (UPGMA) and dendrogram illustrated that Harri and Aradi goat breeds were grouped to gather where Najdi breed was group apart. Thus ISSR markers provide good insight into the genetic diversity available across and within Saudi goat breeds. It also could be concluded that Najdi, Harri and Aradi Saudi goat breeds possess the needed amount of genetic variation required for further genetic improvement of these breeds.
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