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۱Immobilization of Acidithiobacillus ferrooxidans on monolithic packing for biooxidation of ferrous iron
اطلاعات انتشار: Iranian Journal of Biotechnology، ششم،شماره۳(پياپي ۲۳)، ۲۰۰۸، سال
تعداد صفحات: ۷
The oxidation of ferrous iron (Fe2+) in solution using Acidithiobacillus ferrooxidans has industrial applications exclusively in the regeneration of ferric iron (Fe3+) as an oxidizing agent for the removal of hydrogen sulfide from waste gases, desulfurization of coal, leaching of non–ferrous metallic sulfides and treatment of acid mine drainage. The aim of this investigation was to increase the bio–oxidation rate of ferrous sulfate by using immobilized cells. Rate of Fe2+ oxidation was determined in a packed–bed bioreactor configuration with monolithic particles being used as support material.Biooxidation of ferrous iron by immobilized cells was investigated in repeated batch culture and continuous operation using a laboratory scale packed–bed bioreactor. On this account, effects of process variables such as dilution rate and initial concentrations of Fe2+ on oxidation of ferrous sulfate were consequently investigated. During repeated batch culture, the immobilized–cells were stable and showed high constant iron–oxidizing activities. A maximum Fe2+ oxidation rate of 6.7 g\l\h was achieved at the dilution rate of 2 h–1, while no obvious precipitate was detected in the bioreactor.
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