توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱In Vitro Biocompatibility of Monkey Epithelial Kidney Cells (Vero) on Poly (3–Hydroxybutyrate) Nanofibreous Scaffolds
اطلاعات انتشار: اولین کنفرانس ملی علوم و فناوری نانو، سال
تعداد صفحات: ۴
Poly (3–hydroxybutyrate) (PHB) is contemplated as proper naturally biodegradable polyester for medical applications. In this study, we fabricated the PHB scaffolds through electrospinning and evaluate behavior of monkey epithelial kidney cells (Vero) on these scaffolds by means of MTSassay, staining and Scanning Electron Microscopy (SEM) photographs. Our results show that, these scaffolds had not any toxicity effect on cells because Vero cells can proliferate on scaffolds shown by MTS assay and staining with DAPI<\div>

۲Towards the development of a bioengineered scaffold: Comparison of different protocols for human nasal septum cartilage decellularization
نویسنده(ها): ،
اطلاعات انتشار: نخستین کنفرانس ملی دستاوردهای نوین در علوم زیستی و کشاورزی، سال
تعداد صفحات: ۶
Biological and biomimetic scaffolds derived from the decellularized extracellular matrix (ECM) of intact mammalian tissues have been successfully used in a variety of tissue reconstruction methods. There are a number of techniques to decellularize a tissue that generally fall into the categories of physical, chemical, and enzymatic, and these techniques are necessarily used in combination.The objective of this study was to examine four different decellularization techniques, including Triton X–100, sodium dodecyl sulfate (SDS) and nuclease solutions on human nasal septum cartilage (hNSC) constructs.Histological analysis showed, after decellularization with SDS, cells lacunae of all treated cartilage samples were empty, and no cell nuclei were left. Additionally, Masson’s trichrom staining results demonstrated a very intense blue staining of the collagen matrix of all examined decellularized samples, as well as native cartilage tissue. Overall, treatment with 2% SDS and nuclease solution for 6hr appeared to be the most effective agent for cartilage decellularization, as it resulted in complete decellularization while maintaining the collagen matrix. The results of this study are exciting as they indicate the feasibility of creating engineered cartilage that may be non–immunogenic as a replacement tissue.<\div>
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