توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Effects of dichloromethane extract from Brittle star (Ophiocoma erinaceus) on differentiation of B16F10 melanoma cells
نویسنده(ها): ، ،
اطلاعات انتشار: سومین کنفرانس بین المللی دستاوردهای نوین در علوم مهندسی و پایه، سال
تعداد صفحات: ۱۰
Melanoma is one of the most dangerous skin cancers, and its high mortality rates and lack of response to chemotherapy are the main challenges to its treatment. Many investigations have sought an anti–cancer treatment of a natural origin with the ability to control the melanoma cells to promote differentiation into the adult cells and inhibits of cell proliferation. The current study investigated the effects of dichloromethane extract taken from Brittle star (Ophiocoma erinaceuss) on the cancerous cells of B16F10 melanoma. B16F10 cells were cultivated in an RPMI 1640 culture environment. After 24 hours, they were treated with different concentrations of dichloromethane extract from Brittle star for periods of 24 and 48 hours. The distinctive effects on the cells were studied by reverse microscope, melanin test, and protoporphyrin IX test, and expression of the microphethalmia factor (MITF) and cytotoxic effects were assessed by MTT assessment and apoptosis analysis by flow cytometry. The morphological studies of cells, MTT test, and analysis of the cell cycle showed that doses less than 31 µg\ml of dichloromethane extracted from Brittle star could control the multiplication and distinction of melanoma cells, and higher doses could even result in their mortality. Investigations of cell marker differentiation increased melanin production, and protoporphyrin IX and MITF expression all showed differential effects of dichloromethane extract. Dichloromethane extracted from Brittle star (Ophiocoma erinaceus) can control the multiplication and differentiation of melanoma cells and can therefore be an appropriate candidate for anti–tumor studies.<\div>

۲Cytotoxic evaluation of different fractions of Salvia chorassanica Bunge on MCF–7 and DU 145 cell lines
اطلاعات انتشار: Research in Pharmaceutical Sciences، يازدهم،شماره۱(پياپي ۳۴)، ۲۰۱۶، سال
تعداد صفحات: ۸
Because of antimicrobial, antioxidant, and anticancer potential, Salvia chorassanica Bunge (Lamiaceae) has been considered as a popular herb in Iranian traditional medicine. Previous studies have shown remarkable cytotoxic properties of the methanol, n–hexane and dichloromethane extract of S. chorassanica on human cervical cancer cells. To seek the therapeutic potentials of S. chorassanica, this study was undertaken to evaluate the cytotoxic activities of various extracts of this plant on human breast MCF–7 and prostate cancer DU 145 cells. The DU 145 cells were exposed to different concentrations of plant extracts (1–200 µg\ml). Cytotoxic activities were examined using alamarBlue® assay and apoptosis was assessed by acridine orange\propodium iodide double staining and evaluation of DNA fragmentation by flow cytometry. Our findings indicated that n–hexane and dichloromethane extracts had more cytotoxic activities against DU 145 and MCF–7 cell lines compared with other extracts (P0.05). The acridine orange\propodium iodide staining showed apoptogenic properties of n–hexane and dichloromethane extracts which was consequently confirmed by flow cytometric histogram that exhibited an increase in sub–G1 peak in treated cells as compared with untreated cancer cell lines. Taken together, these observations demonstrated cytotoxic effects of S. chorassanica extracts on MCF–7 and DU 145 cell lines which is most likely exerted via apoptosis cell death. Therefore, further investigations on S. chorassanica extracts as potential chemotherapeutic agents are warranted.

۳Effect of Holothuria leucospilota extracted saponin on maturation of mice oocyte and granulosa cells
اطلاعات انتشار: Research in Pharmaceutical Sciences، يازدهم،شماره۲(پياپي ۳۵)، ۲۰۱۶، سال
تعداد صفحات: ۸
Sea cucumbers saponins are triterpenoid glycosides which exert beneficial biomedical effects. This study was performed to assess the effect of saponin extracted from sea cucumber Holothuria leucospilota (H. leucospilota) on maturation of mice oocytes and granulosa cells. The germinal vesicles oocytes were collected from 6–8 weeks old Naval Medical Research Institute (NMRI) mice ovaries, randomly divided into untreated and four experimental groups and cultured In vitro. Maturation medium was supplemented with 0, 1, 2, 4 and 8 μg\ml saponin for 12 days. The rates of maturation were recorded through morphological observation by measurement of follicle diameter during treatment. After 4 days, the effects of saponin on granulosa cells were investigated by reactive oxygen species (ROS) measurement, super oxide dismutase (SOD) activity, caspase assay and tumor necrosis factor–alpha (TNF–α) expression. The oocyte maturation rate was significantly higher in treated groups (1 µg\ml). The ROS and SOD assays demonstrated the antioxidant potential of saponin. The caspase assay exhibited that optimum concentrations of saponin (1, 2 µg\ml) reduced caspase activity in granulosa cells. Flow cytometry showed that optimum concentration of saponin promoted oocyte maturation via down regulation of TNF–α as follicular degenerative factor in nursing cells. These results proposed that maturation rate were obtained after the incorporation of 1 µg\ml sea cucumber saponin. Moreover, the extracted saponin at concentrations of 1, 2 µg\ml enhanced follicle growth which is accompanied by attenuating ROS formation, elevating SOD activity and reducing TNF–α expression in granulosa cells. But, further examinations are required to understand precise mechanisms of saponin action on oocyte and granulosa cells.

۴Anticancer Properties of Chrysin on Colon Cancer Cells, In vitro and In vivo with Modulation of Caspase–3, –9, Bax and Sall4
نویسنده(ها): ، ،
اطلاعات انتشار: Iranian Journal of Biotechnology، چهاردهم،شماره۳(پياپي ۵۵)، ۲۰۱۶، سال
تعداد صفحات: ۸
Background: The SALL4\Sall4 is constitutively expressed in human and mice. SALL4 mRNA could be used as a marker for the diagnosis of different types of cancers. On the other hand, chrysin has diverse biological properties. Objectives: In the present study, the effect of the chrysin was investigated on the CT26 colon cancer in vitro and in vivo. Furthermore, the expression levels of the stem cell markers; sall4 and Bax was analyzed, as well. Materials and Methods: The cytotoxic effects and the type of cell death induced by chrysin were evaluated using a number of biological assays. The apoptotic pathway was examined by caspase–3and caspase–9 assay. The in vivo antitumor efficacy of chrysin on transplanted CT26 tumor cells in BALB\c mice was investigated. In addition, mRNA expression of sall4, Bax was analyzed with RT–PCR. Results: MTT assay and morphological characteristics showed that chrysin exerted a cytotoxic effect on CT26 cells in a dose dependent manner with IC50= 80 mg.mL–1. The biological assays have indicated that chrysin administrated cytotoxicity on colon cancer cells through recruitment of the apoptosis. Caspase–3 and caspase–9 colorimetric assays, in addition to Bax expression analysis, have indicated the involvement of intrinsic apoptotic pathway in the cytotoxic effect of the chrysin. The in vivo assay revealed a remarkable reduction of the colon tumor volume in treated mice (8, 10 mg.kg –1) as compared to the untreated mice. RT–PCR elucidated that chrysin attenuated tumor volume through down regulation of the sall4 and up–regulation of the Bax. Conclusions: It was demonstrated that chrysin accomplishes anti–cancer effect on colon cancer cells via induction of the apoptosis and attenuation of the sall4 the expression. These findings introduce chrysin as an efficient apoptosis based therapeutic agent against colon cancer.
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