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۱PCR–based markers for identification of some allelic variation at Glu–1 and Glu–3 loci in common wheat
اطلاعات انتشار: Journal of Plant Molecular Breeding، دوم،شماره۲، ۲۰۱۴، سال
تعداد صفحات: ۱۵
Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW\LMW–GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern using SDS–PAGE and their PCR based on DNA markers in 60 advanced wheat lines and cultivars with different origins. Specific DNA markers regarding to Glu–1 loci, such as 1319 bp, 669 bp and 450 bp fragments were respectively validated for 2*, 17+18, 5+10 alleles. These alleles showed the highest allelic percentage in Glu–1 loci in studied cultivars. However the Null, 7+8 and 5+10 alleles showed the highest allelic percentage in advanced lines. In this study, 23%, 40% and 37% of cultivars respectively, got good (10), moderate (8–9) and weak (4–7) quality scores. In advanced lines, 18%, 44% and 38% got good, moderate and weak quality scores respectively. Ten specific DNA PCR markers were also detected for genotyping Glu–B3 alleles. The most frequent Glu–B3 alleles in wheat cultivars were i, a, b and d with 24%, 21%, 20% and 12%, respectively.Specific PCR markers regarding to the reported Glu–B3 alleles were produced as 621bp, 1095bp, 1570 bp and 662bp consequently. The most frequent Glu–B3 alleles in advanced lines belonged to a, i and d alleles with 35%, 26% and 21% respectively. The results provided useful information for breeding program to improve breadmaking quality and develop new cultivars.
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