توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Optimization of secretory expression of recombinant hGM–CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method
اطلاعات انتشار: Iranian Journal of Biotechnology، دهم،شماره۴(پياپي ۴۰)، ۲۰۱۲، سال
تعداد صفحات: ۶
Human granulocyte macrophage colony stimulating factor (hGM–CSF) has many therapeutic applications. In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration and post–induction time on the secretion of recombinant hGM–CSF into the culture medium by recombinant Escherichia coli during high cell density cultivation were evaluated by using the Taguchi statistical method. The results indicated that glucose, 1mM IPTG and a time of 6 h post–induction, represented optimum conditions. The secreted hGM–CSF, overall volumetric productivity and purified hGM–CSF were 373 mg\l, 18 mg\l\h and 63 mg\l, respectively.
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