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۱Biological and Molecular Studies on TrichodermaViride–Plant Pathogenic Fungi Interactions
اطلاعات انتشار: World Applied Sciences Journal، بيست و يكم،شماره۱۲، ۲۰۱۳، سال
تعداد صفحات: ۸
Trichoderma is extensively used as bioagent against different plant pathogenic fungi. Different plant pathogens were isolated from rotted root and wilted tomato plants cultivated in Alexandria, Beni–Swiff, EL Dakahlya and El– Fayoum governorates in Egypt. From these plant pathogenic fungi; Fusarium oxysporum, Rhizoctonia solani, Fusarium verticilloid, Alternaria alternata, Mucor racemosus and Aspergillus niger were isolated and identified. Antagonistic test was performed between the Trichoderma viride and the above pathogenic fungi to study microbe–microbe interaction. Different inhibition zones were observed and the percentage of the fungal linear growth was ranged from 40 to 80 % compared with the control. The fungal hyphae were collected around the clear zones and subjected to Differential Display –PCR using 5 different arbitrary primers. The effects of the bioagent on the genetic profile of the examined pathogenic fungi were observed as up regulated and down regulated genes. Three of the up regulated genes were selected randomly and subjected to DNA sequence. The sequence analysis of the sequenced genes revealed that the three selected genes are; endogluconase (860 bp), polyketide synthas (508 bp) and FMK1(618 bp) respectively. It was observed that the function of the three analysed genes play an important role either in biocontrol or in fungal defense activity. Trichoderma viride could be used as biocontrol agent for a wide range of plant pathogens if the suitable conditions were serving for this valuable organism.
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