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۱Molecular Diversity of Alpha Toxin Produced by Clostricium perfringens Strains Causing Avian Necrotic Enteritis
اطلاعات انتشار: World Applied Sciences Journal، بيست و يكم،شماره۱، ۲۰۱۳، سال
تعداد صفحات: ۶
NineClostridium perfringens strains were isolated from fifteen intestinal content samples collected from commercial broilers which suffered from necrotic enteritis NE. Molecular typing of C. perfringens was performed by PCR using three sets of primers specific for toxin –producing genes of C. perfringens [alpha (900bp),beta (236bp) and epsilon (541bp)]. Six strains of C. perfringens type A and three strains type D were fully identified. Comparison of alpha toxin genes among C. perfringens type A and D field isolates by nucleotide sequence analysis revealed somewhat similarity between them and published toxin genes of C. perfringens in gene bank with percentage 84 and 75% respectively also dissimilarity between type A and D field isolates was recorded with percentage 54%.
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