توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Induction of polyploidy in citrus rootstocks
اطلاعات انتشار: کنفرانس بین المللی پژوهش های نوین در علوم کشاورزی و محیط زیست، سال
تعداد صفحات: ۷
The present investigation was undertaken to Induction of polyploidy in citrus rootstock through Colchicine Treatment. Seed were immersed in different concentrations of colchicine treatments (0, 0.2%, 0.6%, 1.2%) for different rootstock (Citrange, Citromelo and Sour orange). The seedling ploidy was determined via flow cytometry. Higher colchicine concentrations decreased the survival. Mixoploeid Citrange produced by 0.6% Colchicine treatment. Morphological data confirmed the results of flow cytometry. The mixoploid from Citrange can be used for breeding programs<\div>

۲The effect of growth regulators (NAA and 2,4–D) on callus induction in citrus rootstock
اطلاعات انتشار: کنفرانس بین المللی پژوهش های نوین در علوم کشاورزی و محیط زیست، سال
تعداد صفحات: ۶
The present investigation was undertaken to study the effect of different concentrations and combinations of 2, 4–dichloro–phenoxyacetic acid (2, 4–D), naphthalene acetic acid (NAA) on callus induction of five citrus rootstocks (Sour orange, Citromelo, Citrange, Poncirus, Volkameriana) on MS basal medium. Different concentration of 2,4–D and NAA were tasted in order to obtain the best callus formation. Maximum callus induction response (100%) was obtained on MS medium with 2,4–D (2 and 3mg\l) and NAA (1.5 mg\l) for Volkameriana. Best callus induction response of Citrange (83.33%) was observed on MS medium with 2,4–D (1 mg\l) and NAA (1.5 mg\l). The highest frequency of the callus induction rate for Poncirus and Citromelo (91.67%) was occurred on MS medium supplemented with 2.4–D (3 mg\l) and NAA (1.5 mg\l). MS medium supplemented with 2,4–D (1 mg\l) and NAA (1.5 mg\l) showed maximum callus induction (91.76%) for Sour orange.<\div>

۳Genetic transformation of Tomato with three pathogenesis–related protein genes for increased resistance to Fusarium oxysporum f.sp. lycopersici
اطلاعات انتشار: Journal of Plant Molecular Breeding، دوم،شماره۱، ۲۰۱۴، سال
تعداد صفحات: ۱۱
Fusarium wilt caused by Fusarium oxysporum f.sp. Lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. In order to increase the tolerance to this disease, a triple structure containing PR1, chitinase and glucanase genes controlled by 35S promoter was transferred to tomato. Eight days after planting on pre–culture medium, explants were inoculated by Agrobacterium tumefaciens strain LBA4404 containing the aforementioned plasmid. When the regenerated shoots grew to 2–3 cm, they were cut and transferred to rooting medium.The plantlets were then transferred to pots filled with a soil mixture of peat moss and perlite for further acclimatization. The putative transgenic plant lines were analyzed by multiplex PCR and the transcription of the transgenes was confirmed by RT–PCR method using the specific primers. The estimated value for the frequency of the simultaneous transfer of chitinase, glucanase and PR1 genes to tomato was 2.7%. Protein extracts of transgenic plants expressing chitinase, glucanase and PR1 genes inhibited in vitro hyphal growth of F. oxysporum f.sp. lycopersici. Compared with non–transgenic control plants, despite some alterations in chlorophyll content no other morphological changes were observed in transgenic plants. The total content of chlorophyll “a” and “b” in transgenic plants were 31.8 and 36.2 % higher than that of control plants, respectively, which may be attributed to metabolic changes due to simultaneous expression of three transgenes.
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