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۱production of uricase by a new source Mucor hiemalis
اطلاعات انتشار: چهارمین همایش ملی بیوتکنولوژی ایران، سال
تعداد صفحات: ۱۰
165 Strains of microorganisms with ability to degrade uric were isolated from soil samples during a screeming program. Among them, a zygomucete fungus with well developed columellae showed to produce high levels of the enzyme in a short time. Isolated fungus was identified as Mucor hiemalis according to the morphological and culture characteristics of the organism. The fungus was able to produce an intracellular urate oxidase in a fermentation medium mainly containing uric acid. Optimized composition of the medium consisted of (1 of distilled water) uric acid, 7.0g, maltose, 6.0 g , Vogel stock solution, 20.0 ml and 1.0 ml of 0.5 M copper sulphate.The optimum pH and temperature for uricase production in the optimized medium were pH 6.0 and 30 C, respectively<\div>
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