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۱Comparison of Sorghum (Sorghum bicolor L. (Monech) Genome Sequances with Genome of Rice, Maize and Wheat
نویسنده(ها): ، ، ، ، ،
اطلاعات انتشار: World Applied Sciences Journal، بيست و پنجم،شماره۶، ۲۰۱۳، سال
تعداد صفحات: ۷
Sorghum (Sorghum bicolor L. (Monech) is the genus of family poaceae and is among the one of the most important world's leading cereal crop. A complete sequencing of sorghum genome is a foundation for invigorating progress toward relating sorghum genes comparison with other cereal crops as rice, maize, sorghum and wheat. Total mRNA two sorghum varieties (Henong–16 and Qian–3) was isolated from fresh leaves samples using Trizol reagent and Plant RNA mate (TAKARA). Colony PCR was used for 10 forward and 100 reverse clones were sequenced by two–way hybridation using Mega BACE1000 to obtain better quality of 200 EST sequences. Cross–match software and ClustalW2 were used to obtain vector sequence shielding and multiple comparisons. Using BLAST at NCBI database for homology comparisons with rice, maize and wheat, it was concluded that a number of sequences of sorghum varieties had same degrees of homology with known proteins or genes. Results regarding forward and reverse sequences matching with rice, maize and wheat through BLAST at NCBI database for homology comparisons are given in table 1 and 2. It was noted that 19 sequences out of 100 of sorghum variety “Henong–16” and 12 sequances out of 101 sequances of sorghum variety “Qian–3” had shown matching for different gene descriptions from NCBI database expressing genes for rice, maize and wheat matched with NCBI database. It was concluded that the genome sequnce of sorghum is the useful for the genome and evolutionary studies of rice, maize and wheat.
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