توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Genetic Polymorphism Detection in Four Genes in Egyptian and Saudi Sheep Breeds
نویسنده(ها): ، ،
اطلاعات انتشار: World Applied Sciences Journal، بيست و هفتم،شماره۱، ۲۰۱۳، سال
تعداد صفحات: ۱۱
This work aimed to study the genetic polymorphism in Booroola gene (FecB), Bone morphogenetic protein–15 (BMP–15), Myostatin (MSTN) and Ghrelin genes and its effects on the production. Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) was used to detect polymorphisms of three candidate genes in five Egyptian and Saudi sheep breeds (Barki, Ossimi, Rahmani, Najdi and Harri) to detect the genotype of FecB, BMP–15, MSTN genes and their allele frequencies. Genotypes were determined by PCR amplification followed by single nucleotide polymorphism (SNP) anthor method for ghrelin gene (Barki, Rahmani, Saidi, Najdi and Harri). Results showed that the polymorphism frequencies of FecB gene with Avall digestion are significantly imbalanced in five breeds. The Najdi, Harri, Barki, Rahmani and Ossimi sheeps were all homozygous carriers (BB). PCR–RFLP with HinfI digestion was used to investigate the genotyping of FecXG loci in exon 2 BMP–15 genes. The genotype frequencies for ++ were: 0.32, 0.30, 0.29 from Barki, Ossimi, Rahmani, respectively, while +G genotype were 0.68, 0.70 and 0.71 after digestion with HinfI. Frequencies of allele + were 0.66, 0.65 and 0.64 in Barki, Ossimi, Rahmani, breeds in Egypt, while 0.34, 0.35 and 0.36 for allele G in Barki, Ossimi, Rahmani, breeds. BMP–15 gene was found to be monomorphic in Najdi and Harri Saudi sheep breeds. Myostatin gene was found to be monomorphic with HaeIII digestion in all sheep breeds. All Egyptian and Saudi samples in myostatin locus were digested by HaeIII enzyme and showed the mm genotype, all of them were monomorphic. The sequence analysis of ghrelin amplified PCR product indicated three nucleotide substitutions (T to C; A to G and A to C) at nt 38 and nt49, nt 108, nt 60 and nt 71 in Rahmani. The six different detected variables were present in the Saidi sheep breed at different frequencies with a majority of five transversion and one transition (G to T, A to T, A to T, G to C, G to T) at nt 14, nt 56, nt 58, nt 67, nt 60 and 71, respectively. This is one of the first studies on polymorphism of BMP–15, myostatin and ghrelin genes in Egyptian and Saudi sheep breeds.
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