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۱In–silico identification of microRNAs from expressed sequence tags of vitis vinifera involved in anthocyanins biosynthesis
نویسنده(ها): ، ،
اطلاعات انتشار: اولین کنگره بین المللی و سیزدهمین کنگره ژنتیک ایران، سال
تعداد صفحات: ۴
Plant microRNAs (miRNAs) are RNAs of 20–22 nucleotides in length with sequence complementarities to specific mRNAs that are targeted for either cleavage or translational repression. As it has been reported, miRNAs play critical regulatory roles in plant growth and development. Computational predictions have raised the number of miRNAs in grape significantly using an EST based approach. These kinds of approaches are a combinatorialapproach which is amalgamation of bioinformatics software, databases and literature which can be used to identify new miRNA functionally active inanthocyanin biosynthesis. In this study, the functionality of miRNAs in the regulation of anthocyanin biosynthesis has been investigated. Previouslyrecorded miRNAs related to anthocyanin biosynthesis were obtained from literatures and their sequences were gained from miRBase. The miRNAs homologies were searched in the EST database of Vitis vinifera by Blastn. Then secondary structures of them were evaluated by mfold website, online RNA secondary structure prediction software. Finally the proper secondary structures were studied to identify the potential targets and regulating region. As a result, it was indicated that two miRNAs miR160a and miR2950–5p might be engaged in anthocyanin biosynthesis via influence on Auxin response factors (ARF) and F–box only protein 13–like in which control of interact with four PAL isozymes and mediate their proteolytic turnover via the ubiquitination–26S proteasome pathway.<\div>

۲Analysis of Thaumatin–Like Proteins characteristics and structure in plants and fungi by bioinformatics approaches
نویسنده(ها): ، ،
اطلاعات انتشار: اولین کنگره بین المللی و سیزدهمین کنگره ژنتیک ایران، سال
تعداد صفحات: ۷
Plants naturally produce several classes of defense proteins, called pathogenesis–related (PR) proteins, in response to a wide range of environmental stresses .The PR protein superfamily includes 17 subfamilies designated PR1– PR17. The PR5 proteins, known as Thaumatin like proteins (TLP), have been shown to have antifungal activity against many fungal pathogens. Though, recently they are discovered in fungi and animals as well. There are several mechanisms in which TLPsact as a defense protein like being involved in specific plasma membrane component(s) of the fungal target. In this studysome characteristics of TLPs from monocotyledons, di–cotyledons and even fungi were studied. Amino acid sequences of24 TLPs obtained in FASTA format from Uniprot database. The physicochemical properties and secondary structures ofprotein sequences obtained using ProtParam and Sopma. Alignment and prediction of common secondary structures weredone by CLC software. Phylogenetic trees were drawn by Clustal W. The resulted phylogenic tree showed that Oryza sativa subsp. Japonica out grouped which had out range of isoelectric pH and Rhizoctonia solani stand in an out–group from other fungi. Random coil and then extended strand had more percentage of secondary structures. CLC main workbench analysis revealed a pattern length of 9 residues Sequence as DVSLVDGFN. Archschema analysis showed PF00314 as main Pfam domain of Thaumatin family, which was common in Ripening–associated protein Glucan endo–1, 3–beta–glucosidaseand some defense proteins. There were 53 domain of architecture related to TLP such as protein kinase, protein tyrosin kinase, probable lipid transfer, gag polypeptide of LTR copia–type too.<\div>
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