مقالههای M Afsharnasab *
توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقالههای نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده میشوند.
۱Multiple infections in shrimp Litopenaeus vannamei broodstock in commercial hatcheries in Khouzestan Province
اطلاعات انتشار: IRANIAN JOURNAL OF FISHERIES SCIENCES، سيزدهم،شماره۴، Oct ۲۰۱۴، سال ۰
تعداد صفحات: ۱۷
The present study was carried out to investigate the status of health and disease, and their impact on shrimp broodstock (Litopenaeus vannamei) in Khouzestan Province (Choeibde Area) in the south of Iran. From March 2012 until April 2013, about 140 broodstock and 5000 postlarvae (PLs) were collected from hatcheries and grow out farms. Clinical signs of samples were documented in take history forms and then the samples were transported to Iranian South Aquaculture Institute (Ahvaz). Bacterial and fungal studies were carried out on hemolymph, hepatopancreas and gill tissue and then the shrimps were preserved in Davidson Fixative for histopathology. A part of uropods was also preserved in ethyl alcohol for PCR study and detecting three viruses, WSSV, TSV and IMNV. A part of PLs was also preserved in ethyl alcohol for PCR and the remaining was preserved in Davidson Fixative for histopathology. The results showed that 5 bacteria consisting of Vibrio alginolyticus, V. proteolyticus, V. mimicus, A. hydrophila and Plesiomonas shigelloides and one fungi Aspergillus fumigatus were identified. The results of PCR exhibited that the broodstock was free of the three viruses and the PL was infected with WSSV. In histopathology some tissues showed the effects of Vibrio infection in different organs such as gill and midgut, and the infection of PL tissues showed the Cowdry type A inclusion bodies in WSSV. Two specific signs of abnormality were also exhibited in histopathology that we call them Pseudo inclusion and Reolike particles and we need to conduct a new study to clarify detailed information about these finding. This finding can be used for assessing the health of shrimp culture and prevention of disease in broodstock in Iran.
اطلاعات انتشار: IRANIAN JOURNAL OF FISHERIES SCIENCES، چهاردهم،شماره۲، Apr ۲۰۱۵، سال ۰
تعداد صفحات: ۸
The objective of our study was to explain the histopathological changes of monodon baculovirus (MBV) in hepatopancreas and midgut tissues of the cultured Penaeus vannamei. Five–hundred and forty juvenile of P. vannamei with average size of 7.99±0.54 g and 3600 post larvae 10–15 were distributed to 18 glass aquariums (50×50×60cm) with 100L well aerated water per each aquarium as water borne MBV (group A) and food borne MBV (Group B) and one control group (C), in triplicates. Also, 3600 post larvae were dedicated for water borne exposure (D) based on one time immersion exposure in 24 h without water exchange and the untreated group was studied as control in triplicates. The specific pathological sign of MBV was observed as a multiple intranuclear eosinophilic occlusion bodies (OBs) in hepatopancreas and midgut tissues. Our result indicates that the severity of the MBV infection is more considerable in post larvae than juvenile stage and confirms that MBV can be an invasive pathogen for shrimp culture industry in Iran.
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