توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Detection of amplified SRY gene by a novel electrochemical biosensor based on gold nanoparticles
نویسنده(ها): ، ، ، ، ،
اطلاعات انتشار: Scientia Iranica، نوزدهم،شماره۳، ۲۰۱۲، سال
تعداد صفحات: ۶
This paper describes an electrochemical hybridization biosensor that investigates DNA hybridization on the modified Glassy Carbon Electrode (GCE). GCE was modified by a mixture of Au nanoparticles (AuNPs) and calf thymus DNA (ctDNA). SH–ssDNA was self–assembled on a modified electrode, which is a segment of the sex determining region of the Y (SRY) gene. This electrode can detect the complementary and non–complementary strand of SRY gene, by Methylene Blue (MB), as an electroactive label, and the Differential Pulse Voltammetry (DPV) technique. The modified GCE for the complementary DNA had a linear dynamic range of 0.5–30.0 ppm. This is the first report of sex determination by the electrochemical biosensor of a Polymerase Chain Reaction (PCR) product. Also, this biosensor showed stability on thermal denaturation for five sequential denaturation processes.

۲The effect of pomegranate juice as a natural antioxidant to prevent DNA damages is detectable by application of electrochemical methods
اطلاعات انتشار: Scientia Iranica، بيستم،شماره۳، ۲۰۱۳، سال
تعداد صفحات: ۵
The effects of ascorbic acid concentration in pomegranate juice as an antioxidant and its bi–functional role in Fenton reaction was investigated. Hydroxyl radical (OH.) produced through Fenton reaction plays a major role in the formation of DNA oxidative damage. The study of DNA damage by Fenton reaction was performed using Glassy Carbon Electrode (GCE). The damaged DNA is adsorbed on the electrochemically modified GCE, and results in oxidative peaks corresponding to the oxidized adenine and guanine residues in DNA. The effects of hydroxyl–radical scavenger (glucose), Fe2+ as reactant and EDTA as iron chelator on the DNA damage were also investigated. The proposed electrochemical method can be used for the estimation of DNA oxidative damage from a new point of view.
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