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۱Genetic Variability Among Three Sweet Basil (Ocimum basilicum L.) Varieties as Revealed by Morphological Traits and RAPD Markers
نویسنده(ها): ، ،
اطلاعات انتشار: World Applied Sciences Journal، بيست و چهارم،شماره۱۱، ۲۰۱۳، سال
تعداد صفحات: ۹
Basil is used as culinary herb and assigned as medicine and spice plant. In order to evaluate somemorphological variability and RAPD analysis for herb growth and oil yield characters among three varieties (French، Purple and Lemon basils)، an experiment was conducted in two seasons (2010–2011) over two cuts in complete randomized blocks design. The analysis of variance showed highly significant differences among varieties and cuts in studied characters. Ranges of herb dry yield were (68.40–86.30g)، oil percent (2.30–2.90 ml.) and oil yield (1.22–2.24 ml.). The highest values of genotypic coefficients of variation (G.C.V %)، genetic advance (GA) and broad sense heritability (h b) were obtained for stem d 2 ry weight، linear growth، herb dry weight and leaf dry weight. The study reflected the importance of herb dry yield and essential oil percent as selection criteria for improvement oil yield in sweet basil. Varieties were analyzed through RAPD markers with five random primers to determine the extent of molecular characterization. The results showed 58 amplified fragments which ranged from 2010 to 80 bp.، 26 of them were polymorphic (44.83 %) and the other 32 amplified were monomorphic. Our observations suggested that morphological variability and RAPD analysis could help in identifying genetic variations among different varieties of basil، help in plant improvement and develop a well–organized way to conserve the genetic wealth of basil varieties.

۲Assessment of Genetic Variability for Three Coriander (Coriandrum sativum L.) Cultivars Grown in Egypt, Using morphological Characters, Essential Oil Composition and ISSR Markers
نویسنده(ها): ، ، ، ، ،
اطلاعات انتشار: World Applied Sciences Journal، بيست و پنجم،شماره۶، ۲۰۱۳، سال
تعداد صفحات: ۱۱
Coriander (Coriandrum sativum L.) is an annual spice herb that belongs to Apiaceae (Umbelliferae) family with widely diversified uses. Genetic variability, essential oil composition and ISSR molecular markers for seven characters in ten selected genotypes within three coriander cultivars were studied. Field experiment was laid out in a randomized complete blocks design with three replications. The analysis of variance revealed that there were highly significant (p0.01) differences among and within coriander cultivars for all the characters, except total number of branches which showed significant (p0.05) among the genotypes. Heritability and genetic advance were high for fruit yield, linear growth and plant height in selected followed by Russian and Balady cultivars. Essential oil obtained by hydro–distillation was characterized by gas liquid chromatography (GLC) indicated that, ten different compounds were identified in essential oil of all varieties. Linalool, Nerol, Bornel, Gereniol and –Terpinene were identified as main components in the oil of all cultivars. Linalool was identified as the main compound and the maximum value in Russian (69.50) followed by Selected (65.48) and Balady (62.53) cultivars, respectively. ISSR results with five primers revealed 26 amplified fragments, 12 of them were polymorphic (46%). The similarity indices and dendrogram for the genetic distances of ISSR marker revealed that the highest similarity was (71%) between the Russian and Selected cultivars. Meanwhile, the lowest (58%). Similarity index was between the Balady and Russian cultivars. These results can be used in further genetic diversity studies for genotypes identification and characterization which can be helpful in breeding programmes for crop improvement.
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