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۱Screening and evaluation of indigenous bacteria from the Persian Gulf as a probiotic and biocontrol agent against Vibrio harveyi in Litopenaeus vannamei post larvae
اطلاعات انتشار: IRANIAN JOURNAL OF FISHERIES SCIENCES، دوازدهم،شماره۴، oct ۲۰۱۳، سال
تعداد صفحات: ۱۴
Isolation of autochthonous bacteria from marine sources as a potential probiont in biocontroling against pathogenic Vibrio species in the shrimp culture industry was the aim of current research. A total of 198 bacterial strains were isolated from pond water, sediment, hepatopancreas and gut samples of shrimps after culturing the samples on Tryptic Soy Agar and incubated at 30 °C for 24–48 h. The isolates were tested for their antagonistic activity in contact with Vibrio harveyi. Two strains (IS02 and IS03) that isolated from the gut and pond sediment were showed antagonistic against V. harveyi. According to 16S ribosomal DNA gene sequence analysis, the strain IS02 was identified as Bacillus subtilis and IS03 as B.vallismortis. Further, the two bacterial species, B. subtilis and B. vallismortis were challenged separately for probiotic activity in the post larvae of Litopenaeusvannamei against pathogenic V. harveyi. The present study identified B.subtilis IS02 and B.vallismortis IS03 had biocontrol activity against V. harveyiin vitro and in vivo and they increase growth performance of L.vannamei in post larvae stage.

۲Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing
اطلاعات انتشار: IRANIAN JOURNAL OF FISHERIES SCIENCES، سيزدهم،شماره۲، Apr ۲۰۱۴، سال
تعداد صفحات: ۱۰
A Gram–negative, rod–shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery–reared larval of Iran Shrimp Research Centerfollowing mass mortality . Seven isolates of Vibrio spp.were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate– Bile Sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O\129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1).
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