توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Iranian H9N2 Avian Influenza Virus is closely related to Pakistani Parakeet Influenza Virus isolates
اطلاعات انتشار: سال
تعداد صفحات: ۵
Sequence analysis and phylogenetic study of hemagglutinin (HA) gene of H9N2 subtype of avian influenza virus isolates (outbreaks of 1998–2002) in Tehran province (Iran) were studied. PCR products of a 430–bp fragment of 16 isolates were sequenced and then were aligned with the reported sequences in Genbank. Nucleotide sequence comparisons of HA gene from Iranian isolates showed 97–99% identity within the group, and 98% homology with the two isolates [A\Parakeet\Narita\92A\98 (H9N2)] and [A\Parakeet\Chiba\1\97 (H9N2)] from Pakistani parakeets imported to Japan. On the basis of phylogenetic evidence and even sequence comparison of Neuraminidase (N) gene of [A\Parakeet\Chiba\1\97 (H9N2)] and [A\Chicken\Iran\IT\99 (H9N2)](Li,2002) it is proposed that the emergence of H9N2 avian influenza infection in Iran originated in Pakistan. Due to the high percentage of H9N2 homology isolates of Iran with other isolates, namley A\quail\HongKong\G1, in Genbank and based on published reports for high similarity with infecting human H5N1 isolates, it seems that the potential of Iranian avian influenza isolates to infect human should be considered<\div>

۲A novel long Vp1 gene deletion include G–H loop in FMDV Type A–87 vaccine strain of Iran
اطلاعات انتشار: چهارمین همایش ملی بیوتکنولوژی ایران، سال
تعداد صفحات: ۵
The initial step of infectious process of FMD virus is attachment to specific cell surface molecules . The G–H loop contains a highly conserved Arginine–Glycine–Aspartic acid (RGD) sequence has been implicated in receptor binding site . We analyzed the VP1 gene sequence of FMDV Type A–87 vaccine strain of Iran. This vaccine strain was the result of 150 serial cytologic passages. The primary sequence alignment with other sequences derived from GenBank shows 39 nucleotides deletion in vp1 FMDV Type A–87 gene sequence, which is missing of 13aa[RGDLGPLAARTAA]. Because of the natural deletion in this virus subtype the RGD sequence in the G–H loop was lost. In spite of losing RGD sequence, the virus grows on BHK–21 suspension cell culture and shows CPE after 16–18 hrs. The PD50 of 13aa–deleted A–87, indicates a satisfactory immune response in guinea pigs.<\div>
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