مقالههای Syed M. Hasnain*
توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقالههای نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده میشوند.
۱Protein Profiles of Indigenous and Commercial Extracts of Amaranthus Pollen for the Diagnosis of Allergy and Asthma Patients
اطلاعات انتشار: World Applied Sciences Journal، سي و دوم،شماره۱۲، ۲۰۱۴، سال ۰
تعداد صفحات: ۸
Background: Pollen grains from Amaranthus viridis (Av) (Slender amaranth, pigweed, etc.) are known to be allergenic and a potential cause of upper and lower respiratory allergic diseases, but neither A.v. extract nor its pollen grains are available commercially. Materials and Method: Amaranthus spp extracts were prepared from pollen grains of both indigenous and commercial species and SPT was conducted on allergic patients. Protein patterns of seven different types of Amaranthus samples as well as serum samples from patients were analyzed by label–free expression proteomics using liquid chromatography coupled with tandem mass spectrometry (LC\MS\MS). Results: Seven (7) proteins that were significantly differentially expressed and distinctively discriminate between patient and control samples, were identified. Only immunoglobulin heavy constant gamma 2 showed greater than 2–fold higher mean expressions in the patient samples than control the observed differences were significant (p 0.0001). Two of the proteins Ig gamma–2 chain C region and Carboxypeptidase N, polypeptide 2 are potential surrogate markers of allergy and asthma. The antigenic variations within Amaranthus species are likely to identify a precise diagnostic species for selection and choice of extracts for some parts of the world. Conclusion: This study highlights the advantage of proteome analysis of different allergens towards discovery of surrogate biomarkers that may become potentially useful to complement currently existing tests for Allergy and Asthma patients.Abbreviations:SPT (Skin Prick Test) Av (Amaranthus viridis) LC\MS\MS (liquid chromatography coupled with tandem mass spectrometry).
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