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۱Hyper Production of Glucoamylase by Aspergillus oryzae FK–923 under Solid State Fermentation
اطلاعات انتشار: World Applied Sciences Journal، سي ام،شماره۱۱، ۲۰۱۴، سال
تعداد صفحات: ۱۵
Glucoamylase production has been investigated by solid–state fermentation of corn flour by Aspergillus oryzae FK–923. Highest glucoamylase production (5582.4 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on corn flour supplemented with30% (w\w) wheat bran, 1% soluble starch, 0.1% (w\w) urea pH 5.5, 60 % (v\w) initial moisture and 30°C after incubation for 72 hrs using 0.2M phosphate buffer as eluting solvent. Ammonium sulfate 80% (w\w) saturation could recovered 76.4% from total enzyme activity of crude enzyme extract involved in 36.8% of total protein precipitated, rising the specific activity from126.30 U\mg protein present in crude enzyme solution to 262.2 U\mg protein present in ammonium sulfate precipitate. Optimum enzyme activity was observed at 55°C and pH 5.5. Enzyme was applied for hydrolyzing 25 % w\v slurry of potato starchy waste resulting 86% saccharification. Enzymatic hydrolyzate 20% glucose was utilized for bioethanol production by Saccharomyces cerevisiae F–727 yielded 11.4% v\v ethanol and 12gl 1 dry yeast biomass. S. cerevisiae F–707 was grown on enzymatic hydolyzate for baker''s yeast production giving 0.42 g\g utilized glucose. The residual mixture contained unutilized medium and fungal biomass was 42% from original weight was analyzed, showed increase in organic matter (OM) crude protein(CP), ether extract (EE) and dietary crude fiber (CF) from 96.98, 9.81, 3.10 and 7.61% to 98.11, 18.45, 10.13 and 27.07% respectively. On the other hand a decrease was seen in both ash and hemicellulose from 4.01 and 17.54% to 1.89 and12.59%, respectively. The gross energy was increased from 454 to 485 Kcal\100g DM. Enzymes assay showed that the residual fermented substrate loaded with glucoamylase 556, alpha amylase 216, xylanase 224 and celullases (FPase 36 and CMCase 28) U\g. and can be used as concentrates feed for cattle and poultry as a source of protein, energy and feed fortification.
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