توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱Chemical composition and antibacterial activity of Methanolic extract of Artemisia annua L. against plant pathogenic bacteria
اطلاعات انتشار: کنفرانس علوم کشاورزی و محیط زیست، سال
تعداد صفحات: ۷
Natural products from plants have great potential as pesticides sources for controlling Bacterial and fungal diseases of plants. In this study antimicrobial activity of the aerial parts extract of Artemisia annua L. was studied in vitro against five plant pathogenic bacteria, Including: Rathayibacter tritici, Pesudomonas syringae , pv. Syringae, Erwinia amylovora, Xanthomonas campestris and Escherichia coli. The chemical constitutions of this extract were analyzed by GC\MS. GC\MS analysis of the extract lead to identification of Fifty–seven components, representing 89.89% of the extract; Arteannuic acid (32.39%), Eudesma–4 (14%), 11– diene (6.36%), Arteannuin b (5.02%), Artemesia ketone (4.85%), 2–Methoxy–4vinylphenol (Phenol) (4.10%), Germacren–D (3.61%), Caryophyllene (3.16%), Camphor (2.40%) and Caryophyllene oxide (1.11%) being the main components. The extract composition and the observed antibacterial properties show that the aerial parts of Artemisia annua L. can be used as potential antibacterial agents for the control of bacterial diseases in plants.<\div>

۲Improvement of qualitative and quantitative traits in soybean [Glycine Max (L.) Merrill] through gamma irradiation
اطلاعات انتشار: Journal of Plant Molecular Breeding، اول،شماره۱، ۲۰۱۲، سال
تعداد صفحات: ۶
Gamma irradiation was used at different doses (80, 160 and 240 Gy) on Glycine Max (L.) Merrill cv. Hill homogenous seeds. A single suitable M2 plant was selected and evaluated at M3 and M4 along with its parent and three other varieties as control in RCBD experiment in Sari Agricultural Sciences and Natural Resources University’s experimental field. M–80–709 and M–160–3429 mutant lines were significantly shorter (55.05 and 72.04 cm respectively). Their branch numbers were however significantly more (8.70 and 11.53 respectively) compared to the parent cultivar (80.82 cm and 6.10 respectively) and other genotypes (p≤0.05), when calculated on per plant basis. Besides, the M–160–3429 was characterized by highest grain yield and oil content (38.25 g and 19.22% respectively), in compare with its parent (12.73 g and 19.09% respectively) and all others control cultivars (p≤0.05). The M–160–3429 mutant line with high grain yield and oil content accompanied with some other favorite morphological traits can be considered as a new promising line of soybean for future studies. Results from this study suggested that mutation breeding procedures at the ranges of 80 to 160 Gy is a capable me­thod for breeding higher grain yield including increasing the oil content as well.

۳Pattern of DNA cytosine methylation in Aeluropus littoralis during temperature stress
اطلاعات انتشار: Journal of Plant Molecular Breeding، اول،شماره۱، ۲۰۱۲، سال
تعداد صفحات: ۹
DNA methylation as an epigenetic mediator plays the important role in spatial and temporal gene regulation and ensures the stability and the plasticity of organism. In this investigation, methylation sensitive amplification polymorphism (MSAP) were assessed in CCGG sites on a halophytic plant, Aeluropuslittoralis in response to different temperature stresses including freezing, low and high temperatures. A combination of 13 primers were able to produce 500 bands, of which 74%, 20.8% and 5.2% were of type I (non methylated fragments), type II (CpG methylated fragments) and type III (CpCpG methylated fragments), respectively. Among these bands, 130 bands were methylated fragments with the highest occurrence of methylation at CpG internal cytosine. The results showed that up to 2% of all methylated bands were polymorphic, which belonged to types II and III. Highest levels of methylation alternations were detected under high and freezing temperatures. The results suggest that apart from cis regulatory logic plant response to the environmental temperatures may be regulated by methylation of CCGG sites of stress–related loci.

۴Codon bias patterns in photosynthetic genes of halophytic grass Aeluropus littoralis
اطلاعات انتشار: Journal of Plant Molecular Breeding، دوم،شماره۱، ۲۰۱۴، سال
تعداد صفحات: ۹
Codon bias refers to the differences in the frequency of occurrence of synonymous codons in coding DNA. Pattern of codon and optimum codon utilization is significantly different between the lives. This difference is due to the long term function of natural selection and evolution process. Genetics drift, mutation and regulation of gene expression are the main reasons for codon bias. In this study, the codon bias analysis was done on photosynthesis and respiratory related genes of phosphoenolpyruvate carboxylase (PEPC), NADP–malic enzyme (NADP–ME), pyruvate orthophosphate dikinase (PPDK), glycerate kinase (GK) (nuclear genes), rubisco, NADH–dehydrogenase subunit F and cytochrome–C (chloroplast genes) from Aeluropus littoralis plant. Nuclear gene sequences were obtained after partial isolation and for chloroplast genes obtained from nucleotide database. Calculation of codon adaptation index (CAI) showed that studied genes with direct or indirect association with photosynthesis, had high level of gene expression and had also a tendency to optimum codon utilization. The results also showed the difference in codon bias between genes encoded in nucleus and chloroplast for some amino acids.

۵Assessment of seed storage protein composition of six Iranian adopted soybean cultivars [Glycine max (L.) Merrill.]
اطلاعات انتشار: Journal of Plant Molecular Breeding، دوم،شماره۱، ۲۰۱۴، سال
تعداد صفحات: ۱۶
Seed protein quality is an important topic in the production of soybean. The quality of soybean proteins is limited by anti–nutrient proteins and low levels of essential sulfur amino acids. In this study, protein content and solubility of six cultivars were evaluated and seed storage proteins were analyzed using SDS–PAGE and scanning densitometry. The results showed that seed storage protein bands were similar among soybean cultivars. However, concentration of β–conglycinin (7S), glycinin (11S) proteins and related subunits were statistically different among the soybean cultivars. According to the results of this study, 033 and DPX cultivars were characterized by high levels of protein content (42.45 %) and protein solubility (76.58 mg g–1) respectively. Two cultivars DPX and JK were also identified by high 11S\7S ratio (1.39 and 1.43 % respectively). Besides, the JK was considered by the lowest concentration of 7S protein (20.35 %). The results showed that a significant negative correlation existed between protein content and solubility (r= –0.66). A significant and moderate positive correlation was found between acidic and basic subunits with 11S protein (r= 0.72 and 0.47 respectively). The 11S and 7S proteins also showed positive and negative correlation with 11S\7S ratio (r= 0.70 and –0.85 respectively). On the other hand, acidic subunits were characterized by significant positive and negative relationship with 11S\7S ratio and some anti–nutrients protein respectively. Thereupon, these results suggested that the development of new genotypes of soybean with high level of acidic subunits of 11S protein can be notable in increasing seed storage protein quality in soybean breeding programs.

۶Involvement of Cytosine DNA methylation in different developmental stages of Aeluropus littoralis
اطلاعات انتشار: Journal of Plant Molecular Breeding، دوم،شماره۲، ۲۰۱۴، سال
تعداد صفحات: ۱۲
DNA methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. To assess DNA methylation pattern in different developmental stages of Aeluropus littoralis, methylation sensitive amplified polymorphism (MSAP) was used. Methylation and demethylation status at the CCGG recognition site were tracked by two sets of cytosine methylation–sensitive enzymes (MspI and HpaII), which were classified into three types. The percentage of total bands per type I (non methylation), type II (CpG methylation) and type III (CpCpG methylation) fragments were 75.7, 19.4 and 4.9, respectively. The most frequent methylation events (19.4%) were observed in type II fragment in which full methylation pattern occurred. Out of 480 bands, 33 bands showed methylation alterations between differential developmental stages in all three types of detectable methylation levels. In this study, polymorphic bands had two main directions associated with methylation or demethylation patterns in which methylation level increased during plant development. The methylation and demethylation events at CG sites could be related to developmental stage–specific gene regulation.
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