توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقاله‌های نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده می‌شوند.
۱The impact of extracellular enzymes of Trichoderma viride and Trichoderma harzianum on succinoglycan produced from Agrobacterium radiobacter
نویسنده(ها): ، ، ،
اطلاعات انتشار: کنفرانس علوم کشاورزی و محیط زیست، سال
تعداد صفحات: ۱۰
Two strain of Trichoderma (T. viride and T. harzianum) were isolated and used for β–glucanase enzyme production. The succinoglycan was produced by fermentation of sucrose by Agrobacterium radiobacter and its chemical structural properties were investigated by TLC, FT–IR and 1H–NMR spectroscopy. The results showed that the biogum is composed of glucose and galactose units, carrying pyruvate, succinate and acetate groups and indicated the presence of succinoglycan. The highest β–glucanase activity was observed in T. harzianum. The SDS–PAGE profiles have several enzyme bonds such as β(1,3), β(1,4) and β(1,6) glucanase. The T. harzianum and T. viride have both enzyme bonds of β(1,3) glucanase and β(1,6) glucanase. Cel6A (CBH II) only was observed in T. harzianum. The high values β–succinoglycanase activity in T. harzianum due to present of β(1,3), β(1,6) glucanse enzymes and synergism that occurs between of them. Succinoglycan is a good substrate for total β–glucanase activity assay.<\div>

۲The synergistic interactions of Trichoderma spp. cellulase enzyme activities in biomass conversion; Part1: comparison of cellulose Iα, Iβ and III
نویسنده(ها): ، ، ،
اطلاعات انتشار: کنفرانس علوم کشاورزی و محیط زیست، سال
تعداد صفحات: ۱۱
Three strain of Trichoderma (T. reesei, T. viride and T. harzianum) were used for cellulase enzyme production. The Avicel, CMC, BC and Whatman NO.1 filter paper, were used to cellulase activity assay. The carboxy methyl cellulose (cellulose III, CMC) and bacterial cellulose (cellulose Iα, BC) were produced by chemical modification of Avicel (cellulose Iβ) and fermentation of sucrose by G. xylinus, respectively. Their chemical structural properties were investigated by FT–IR, XRD and SEM in compare with Avicel. The molecular weight of cellulase enzymes were studied using SDS–PAGE. The lowest extracellular protein production was observed in T. reesei, T. harzianum and T. viride, respectively. The SEM image of substrates, showed more delicacy of BC fibers relative to Avicel and CMC. The diameter ratio of BC to Avicel is approximately 1\30 or less. The FTIR spectroscopy and XRD assessments designated that the produced CMC is carrying a carboxylic group and its crystallinity is 81.84. The crystallinity of the Avicel and BC were demonstrated that the crystallinity index of Avicel (89.15%) was more than that of bacterial cellulose (66.44%). The highest Endo–glucanase (CMCase), Exo–glucanase (Avicelase) and total cellulase (BCase and FPase) activity were observed in T. reesei, T.viride and T.harzianum, respectively. The high Avicelase activity in T. viride due to high produced extracellular protein concentration. The SDS–PAGE profiles have several enzyme bonds such as CBHs, EGs and BGLs. The T. harzianum and T. reesei have both enzyme bonds of Cel6A (CBHII) and Cel7A (CBHI), but T. viride has only a sharp enzyme bonds correlate to Cel7A. Cel12A (EGIII) only was observed in T. reesei. The highest synergy was observed in FPase and BCase of T. harzianum, that containing of large amount of CBH I and CBH II and minor amount of EG. Also, the Cel 3A (BGL I) and CEL 1A (BGL II) were observed for T. reesei and T. harzianum SDS–PAGE profiles, respectively. Finally, the results showed that bacterial cellulose is a good substrate for total cellulase activity assay, because it has the highest enzyme activity in compare of filter paper and avicel. The high values BCase and FPase activites in T. harzianum and T. reesei due to present of CBH and EG enzymes and synergism that occurs between the CBH and EG.<\div>
نمایش نتایج ۱ تا ۲ از میان ۲ نتیجه