Protection of mild steel (UNS G 1020) against microbial induced corrosion using biofilm– producing bacterium Bacillus sp. strain TB16.چهاردهمین همایش بین المللی نفت، گاز و پتروشیمی
A biofilm producing bacterium , strain TB16 was isolated from the tabriz refinerys cooling towers and identified as bacillus sp.by 16SrRNA gene sequencing , shown to be able to reduce corrosion rates of mild steel by establishment of antimicrobial agent producing biofilm which inhibit the activity of sulfate –reducing bacteria SRBs. the corrosion behavior of mild steel UNS1020 was studied during exposure to SRBs in postgates E and \ or luria bertani LB–Bushnel hass BH media in sterile condition in bench scale batch reactor iusing electrochemical impedance spectroscopy EIS. when a mixed SRB culture was intorduced to a non –antimicrobial producing control biofilm of paenibacilus polymyxa ATCC10401 , an increased corrosion was observed and the mild steel coupons underwent more severe corrosion than when only SRB population was present , showing 3 fold increase via EIS. however , when a gramisidin –s–producing bacillus migulans aneurinbacillus ATCC9999 or strain TB16 biofilms were pre –established on mild steel the metal coupons were protected aginst the attack of mixed SRB populations.<\div>
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