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۱Evaluation the Efficacy of Egyptian Propolis Against Parapox Viruses by Production of IFN– , TNF– and Immunoglobulin in Experimental Rat
اطلاعات انتشار: World Applied Sciences Journal، سي و يكم،شماره۲، ۲۰۱۴، سال
تعداد صفحات: ۹
Propolis is a natural product extensively used in food and beverages to improve health and to prevent diseases, showing immunomodulatory properties. The goal of the present study was to evaluate the efficacy of Egyptian propolis (ethanolic and water extracts) against parapox viruses. Twenty–five Albino rats, 250–300 g body weight, were randomly divided into five equal groups. G1 was injected with phosphate buffer saline (PBS) mixed with propolis extract (dose 50 mg \kbw) and kept as control. G2 (control positive rats) were inoculated with PPV isolated field virus. Rats of G3 were inoculated PPV mixed with water propolis extract one dose only. G4 was injected PPV mixed with ethanol propolis extract one dose only. G5 inoculated PPV mixed with ethanol propolis extract two dose two weeks apart. The route of inoculation was subcutaneous (SC) and intradermal (ID) for each rat in all groups. Results revealed that Effect of 5– 20% propolis extract concentration on Embryonated Chicken Egg (ECE) and rats found safe, no effect in the nature and color of embryo fluid comparing with control. Water and ethanol propolis extract caused reduction of parapox viruses infectivity titer from (10 to 10 ) and (10 to 10 ) for reference and isolated parapox viruses 5.3 2.9 4.62 2.3 respectively. The group inoculated with propolis without virus showed slightly suppressed cytokines (TNF– and IFN ) in the rat sera when comparing to control negative rats. Whereas the cytokine production was strongly stimulate significant increase (P 0.05) in sera of rat receiving propolis together with PPV. The highest production of IFN– and TNF– (0.536±0.095, 0.406±0.079 and 0.341±0.052) and (0.295 ± 0.065, 0.253 ± 0.05 and 0.267 ± 0.055) in G5, G4 and G3 measured by ELISA (Means ODs ± SD), respectively. Control positive infected rats with parapox viruses without propolis showed signs of the inflammation and swelling at the site of injection after the 1st week post infection and on necropsy showed congested with petichial hemorrhage in liver, spleen and kidney. Histopathological studies showed acute necrotic hepatitis accompanied with disseminated intravascular coagulopathy (DIC), which is a pathognomonic process. The present study proved that the propolis extract have many effects on the TNF– and IFN– cytokines and humoral immune response may be exploited for the development of effective natural antiviral and immunostimulant product uses in human and animals.
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