مقالههای Ahmad Reza Bahrami
توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقالههای نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده میشوند.
۱Investigating the effect of the methanolic extract from the leaves of Achillea eriophora D. C. on human cutaneous wound healing in an in vitro cellular model
اطلاعات انتشار: کنفرانس بین المللی پژوهش در علوم و تکنولوژی، سال ۱۳۹۴
تعداد صفحات: ۱۳
Achillea eriophora D. C. (Asteraceae), an endemic species of Iran, is used extensively in Iranian folk medicine to treat various ailments, including gastrointestinal disorders, skin inflammations and wound healing. Present study aimed on investigation of the effects of the methanolic extract from the leaves of A. eriophora on stimulation of fibroblast cell proliferation and migration as two pivotal stages in wound healing process. Methanolic extract was prepared by maceration method. It's total phenol and flavonoid contents were measured using spectrophotometry. Cytotoxic and proliferation effects of the extract were evaluated by MTT assay on human fibroblast cells (HFF3). Moreover, scratch assay was used for evaluation of cell migration. In this method, the closure of a denuded area, made by scratching on the confluent monolayer cultures, were measured by microscopy and software analysis.MTT results indicated that the methanolic extract did not have any cytotoxic effect on HFF3 cells when used at concentrations up to 2 μg\ml. Human fibroblast proliferation was stimulated by low concentrations (0.1–0.8 μg\ml) of the extract, and the most effective dose was estimated as 0.1 μg\ml. Migration of the cells was induced by intermediate concentrations (1–30 μg\ml) of the extract. The highest level of migration was observed in the lowest treatment (1 μg\ml). Due to the fact that lower concentration of the extract, which showed the best proliferation and migration stimulatory effects, were not toxic on HFF3 cells, we tend to recommend the crude methanolic extract from the leaves of Achillea eriophora as a potential source for improving the wound healing activity in skin.<\div>
نویسنده(ها): DorMohammad Kordi–Tamandani، Ahmad Reza Bahrami، Raziye Sabbaghi–ghale–no، Hanieh Soleimani، Tayebe Baranzehi
اطلاعات انتشار: Molecular Biology Research Communications، پنجم،شماره۱، ۲۰۱۶، سال ۰
تعداد صفحات: ۴
Recently, inflammation has been found to be a significant factor in the development of Schizophrenia (SCZ). The aim of the present research was to investigate whether interleukin–33 (IL–33, OMIM: 608678) gene polymorphism (rs11792633, C\T) is associated with the development of SCZ or not.DNA was isolated from the serum of 70 patients with SCZ and 70 healthy controls. The PCR based method was used for detection of the IL–33 polymorphism. The CT (OR=0.05, 95% CI: 0.003–0.057, P0.001) andTT(OR=0.12, 95% CI: 0.028–0.46, P0.001) genotypes significantly decreased the risk of SCZ. Our present findings indicate that the IL–33 polymorphism associated with the risk of SCZ.
۳Comparison of pepck gene expression in developing seeds and leaves of chickpea (Cicer arietinum L.) plant (انگلیسی)
نویسنده(ها): Maria Beihaghi، Ahmad Reza Bahrami، Abdolreza Bagheri، Richard Leegood، Mehdi Ghabooli، Jafar Zolala، Farajollah Shahriari
اطلاعات انتشار: دو فصلنامه پژوهش هاي سلولي و ملكولي، اول،شماره۲، ۲۰۰۹، سال ۰
تعداد صفحات: ۷
Phosphoenolpyruvate Carboxykinase, encoded by the pepck gene, plays an important role in gluconeogenesis. It also seems to be important in metabolism of nitrogenous compounds in developing seeds of legumes, including amides and ureides which are then transformed into amino acids, necessary for the synthesis of storage proteins. In this research, pepck gene expression in mRNA level, in different genotypes of chickpea (Cicer arietinum L.), was determined. Two low protein genotypes (MCC291 and MCC373) and two high protein genotypes (MCC458 and MCC053) out of 20 chickpea genotypes were selected. Total RNA were extracted through different stages of seed development, and the expression of the pepck gene was estimated by semi–quantitative RT–PCR. The results of the RT–PCR showed that two isoforms of this gene are expressed in high protein genotypes, whereas in the low protein genotypes, the expression of these isoforms was not obvious. Also this method showed a differential expression of pepck gene in different stages of flowering and seed development. pepck gene is expressed in higher levels during the sheet formation and developing seeds compared to the flowering and seed formation stages. Probably, the differential expression of pepck gene is related to its possible role in metabolism of seed components, particularly in determination of the protein content of chickpea seeds.
۴Cytotoxic effect of essential oils from Salvia leriifolia Benth. on human Transitional Cell Carcinoma (TCC) and mouse fibroblast
نویسنده(ها): Ahmad Reza Bahrami
اطلاعات انتشار: Journal of Cell and Molecular Research، سوم،شماره۲، ۲۰۱۱، سال ۰
تعداد صفحات: ۶
Essential oils, with plant origin, have been of special attention in cancer research during recent years. Despite many reports on cytotoxic effects of plants from genus salvia, the potential application of their extracts in cancer therapy remains to be assessed in more precise and detailed examinations on the main cause of such effects. In this research, the cytotoxic effect and anticancer activity of essential oils from S. leriifolia on human Transitional Cell Carcinomaa (TCC) were studied in vitro. The antiproliferative activity of essential oils on TCC and L929 (control) cells was determined by 3–[4, 5–dimethylthiazol–2–yl]–2, 5–diphenyl tetrazolium bromide (MTT) assay, by which the mitochondrial dehydrogenase enzyme activity is assessed based on reduction of the MTT to purple. The amount of essential oils to induce 50% of cells to die, designated as IC50, was determined by repeated experiments and application of different doses of the essence. The established IC50 on TCC cells for the essences extracted in two different years of 2006 and 2008 and from two locations of Bajestan and Neyshabour was respectively as: 466 and 250 μg\ml, and 233 and 212 μg\ml. S. leriifolia essential oil did not show any detectable effect on L929 cells in this range of concentration. S. leriifolia essential oil has inhibitory effects on the growth of both TCC and normal L929 cell lines, although the effective concentrations were significantly different in these cell lines. This effect was dose dependent.
۵Designing a SYBR Green Absolute Real time PCR Assay for Specific Detection and Quantification of Bacillus subtilis in Dough Used for Bread Making
اطلاعات انتشار: Journal of Cell and Molecular Research، ششم،شماره۲، ۲۰۱۴، سال ۰
تعداد صفحات: ۱۰
In present study, a SYBR green based real time PCR assay was developed for specific detection and quantification of Bacillus subtilis in dough used for bread making. New primer pairs were designed to amplify a 212 base pair fragment of the aprE gene. Specificity of these primer pairs was confirmed with conventional and real time PCR methods. Standard curves constructed using the threshold cycle (CT) versus copy numbers of B. subtilis showed good linearity for reference standards of cloned insert (R2=0.999, slope=–3.035) and also induced contaminated dough (R2=0.988, slope=–3.142), and the melting temperature (Tm=82.2 oC) was consistently specific for the amplicon. Limits of detection were 200 and 2000 colony forming units (CFUs) per ml or g of these samples, respectively. This real time PCR offers a fast tool with high sensitivity and specificity for detection and quantification of this rope–forming pathogen in dough used for bread making.
۶Supplementary Analysis of Phosphoenolpyruvate Carboxykinase Gene Expression in Developing Seeds of Chickpea
اطلاعات انتشار: Journal of Cell and Molecular Research، هفتم،شماره۲، ۲۰۱۵، سال ۰
تعداد صفحات: ۶
Studies on the genes contributing to the seed filling in chickpea and its protein content might be valuable in engineering plants with seeds of a higher nutritional value. A gene of interest is phosphoenolpyruvate carboxykinase (pepck), encoding a protein with a substantial role in the gluconeogenesis pathway. In the present study, the protein content (percentage) was measured in a number of cultivated chickpea genotypes, followed by comparison of the expression levels of pepck gene at different stages of seed filling in some of the genotypes. This study aims at revealing the relation between pepck transcript level with protein content of chickpea seeds which might, in the longer term, end at protein quality improvement of the crop through the gene manipulation procedures. The results which were verified by Real Time–PCR and Western blot techniques in four genotypes of plant, showed that, the amount of pepck expression was significantly higher at the stage of seed fillingthan at other stages in all of genotypes and the lowest levels of expression belonged to flowering and seed formation and the PEPCK protein level was higher in the high protein genotypes compared to the low protein genotypes.
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