مقالههای Waled M. El–Senousy
توجه: محتویات این صفحه به صورت خودکار پردازش شده و مقالههای نویسندگانی با تشابه اسمی، همگی در بخش یکسان نمایش داده میشوند.
۱Molecular Epidemiology of Human Adenoviruses and Rotaviruses as Candidate Viral Indicators in the Egyptian Sewage and Water Samples
اطلاعات انتشار: World Applied Sciences Journal، بيست و هفتم،شماره۱۰، ۲۰۱۳، سال ۰
تعداد صفحات: ۱۳
The prevalence of human rotavirus and adenovirus in sewage and water samples before and after treatment steps and also in clinical samples was compared in this study to determine the most prevalent virus and the more resistant one to treatment processes. The prevalence percentage of rotaviruses in sewage samples collected from raw sewage of Zenin wastewater treatment plant (WWTP) from July 200 to June 2011 was 16.7% (4\24) while the prevalence percentage of adenoviruses in the same raw sewage samples was 91.7% (22\24). The prevalence percentage of rotaviruses in Nile water samples collected from El– Giza water treatment plant (WTP) from July 2009 to June 2011 was 29.2% (7\24) while the prevalence percentage of adenoviruses in the same Nile water samples was 66.7% (16\24). Rotavirus could be only detected and quantified as genome copies and infectious units in the peak months of the virus in autumn and winter. Adenovirus genome copies and infectious units were higher in winter than in the other months but considerable numbers of both of them were quantified all the year. The means of adenovirus genome and infectious units removals were 3.5±1.1 log10 and 2.8±0.8 log10 respectively while the means of rotavirus genome and infectious units removals were 3.75±0.5 log10 and 3±1.2 log10 respectively after treatment processes of Zenin WWTP. The means of adenovirus genome and infectious units removals were 2.3±0.9 log10 and 2.1±0.9 log10 respectively while the means of rotavirus genome and infectious units removals were 2.86±1.1 log10 and 2.57±1.1 log10 respectively after treatment processes of El–Giza WTP. Rotavirus was the most prevalent in diarrheal samples 31.8% (35\110), while adenovirus was detected in 18.1% (20\110) of the samples. Sequence analysis of positive adenovirus samples indicated that 19 samples (6 raw sewage, 5 Nile water and 8 clinical samples) showed similar sequences clustered with human adenovirus 41 strain 503804\KOL\2009 hexon gene with 94% homology and 5 samples 2 raw sewage and 3 Nile water samples) showed similar sequences clustered with human adenovirus 2 strain N377 \TW\05–2 hexon gene with 98% homology. As a conclusion, human adenovirus may be a suitable candidate viral indicator of human viral contamination in Egyptian water and sewage samples.
۲Survival of Adenovirus, Rotavirus, Hepatitis A Virus, Pathogenic Bacteria and Bacterial Indicators in Ground Water
اطلاعات انتشار: World Applied Sciences Journal، بيست و نهم،شماره۳، ۲۰۱۴، سال ۰
تعداد صفحات: ۱۲
The aim of this work is to study the survival of genome and infectious units of adenovirus type 40, rotavirus Wa strain and Hepatitis A virus (HAV) HM 175 adapted strain in Egyptian ground water at different temperatures and their relationships with the persistence of bacterial indicators and pathogenic bacteria. Also, study the effect of limestone on the same viruses and bacteria in ground water. The genome of adenovirus was the more stable and persistent followed by rotavirus Wa strain and finally HAV HM 175 adapted strain. There were gradual decreases in the genome of the three tested viruses parallel with the time. After 12 weeks, adenovirus type 40 genome copies reductions were 0.05 log10, 0.5 log10 and 1.5 log10 at 4°C, 22°C and 35°C respectively. Rotavirus genome copies reductions were 0.1 log10, 0.7 log10 and 2.3 log10 at 4°C 22°C and 35°C, respectively. HAV genome copies reductions were 0.2 log10, 0.8 log10 and 2.7 log10 at 4°C, 22°C and 35°C, respectively. The infectious units of adenovirus were the more stable and persistent than rotavirus Wa strain. There were gradual decreases in the infectious units of the two tested viruses parallel with the time. After 12 weeks, adenovirus infectious units reductions were 0.5 log10, 1.5 log10 and 3 log10 at 4°C, 22°C and 35°C, respectively.Rotavirus infectious units reductions were 0.7 log10, 1.8 log10 and 3.5 log10 at 4°C, 22°C and 35°C, respectively. Higher stability for the genomes of the three tested viruses and the infectious units of adenovirus and rotavirus than E. coli, Enterococcus fecalis, Salmonella spp. and Staphylococcus aureus in ground water at 22°C after 12 weeks was observed. The results showed that, there were gradual decreases. One log10 reduction was recorded after 5 weeks for E coli, Salmonella spp. after 8 weeks for Staphylococcus aureus and after 9 weeks for Enterococcus fecalis when all of them were seeded separately. On the other hand bacterial indicators (E. coli and Enterococcus fecalis) had more survival than pathogenic bacteria (Salmonella Spp. and Staphylococcus aureus). The persistence of viruses and bacterial indicators as well as pathogenic bacteria in ground water treated with limestone was also studied. After 96 hours, the mean reductions of genome copies and infectious units were 0.9 log10, 1.2 log10 and 1.4 log10 for adenovirus type 40, rotavirus Wa strain and HAV HM 175 adapted strain genome copies, respectively and 1.8 log10 and 2.3 log10 for adenovirus type 40 and rotavirus Wa strain infectious units respectively in ground water treated with limestone (6.7 g\l). Also, the results showed that, decrease one log (removal 90%) in flask which contained Salmonella spp. and E. coli treated with limestone was observed after 2 and 4 hours, respectively, the effect of limestone on bacterial indicators and pathogens was faster and higher than the effect on viral genome and infectious units.
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